50227
D -荧光素 钾盐
≥98.0% (HPLC)
别名:
( S )-2-（6-羟基-2-苯并噻唑基）-2-噻唑啉-4-羧酸 钾盐, 4,5-二氢-2-（6-羟基-2-苯并噻唑基）-4-噻唑羧酸 钾盐, 萤火虫荧光素 钾盐
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关于此项目
经验公式（希尔记法）:
C11H7KN2O3S2
化学文摘社编号:
分子量:
318.41
UNSPSC Code:
12352106
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5416262
InChI
1S/C11H8N2O3S2.K/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;/h1-3,7,14H,4H2,(H,15,16);/q;+1/p-1/t7-;/m1./s1
SMILES string
[K+].Oc1ccc2nc(sc2c1)C3=N[C@H](CS3)C([O-])=O
InChI key
UMBKGTQQGYPQBE-OGFXRTJISA-M
assay
≥98.0% (HPLC)
form
powder or crystals
solubility
water: soluble
storage temp.
−20°C
Quality Level
Application
D-萤光素可用作基于荧光素酶(EC 1.13.12.7)的生物发光成像和基于细胞的高通量筛选应用的底物。D-荧光素和萤火虫荧光素酶组成的试剂可以测量ATP。D-荧光素可以作为G蛋白偶联受体-35(GPR35)的部分激动剂使用和研究。
Packaging
无底玻璃瓶。内含物在插入的融合锥体内。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Yimao Zhang et al.
Molecular imaging, 11(6), 499-506 (2012-10-23)
Bioluminescence imaging (BLI) detects light generated by luciferase-mediated oxidation of substrate and is used widely for evaluating transgene expression in cell-based assays and in vivo in relevant preclinical models. The most commonly used luciferase for in vivo applications is firefly
Xinyi Xu et al.
Frontiers in pharmacology, 11, 575-575 (2020-05-21)
Britanin has been reported to have therapeutic effects on neurodegenerative and inflammation-based diseases. However, whether it is involved in the regulation of triple-negative breast cancer development has not been elucidated. In this study, we investigated the anti-tumor activity against triple-negative
Ellen Siebring-van Olst et al.
Journal of biomolecular screening, 18(4), 453-461 (2012-11-01)
The firefly luciferase gene is commonly used in cell-based reporter assays. Convenient luciferase assay reagents for use in high-throughput screening (HTS) are commercially available. However, the high cost of these reagents is not within the means of some academic laboratories.
Yuval Erez et al.
The journal of physical chemistry. A, 116(28), 7452-7461 (2012-06-16)
Optical steady-state and time-resolved spectroscopic methods were used to study the photoprotolytic reaction of oxyluciferin, the active bioluminescence chromophore of the firefly's luciferase-catalyzed reaction. We found that like D-luciferin, the substrate of the firefly bioluminescence reaction, oxyluciferin is a photoacid
Mayu Yasunaga et al.
BMC biotechnology, 17(1), 54-54 (2017-06-24)
Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated treatment of the spheroids with a test object is possible. Generally
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