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Merck
CN

5006

Sigma-Aldrich

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol

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EC 号:
UNSPSC代码:
12352202
NACRES:
NA.75

product name

PureCol, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

质量水平

无菌性

sterile-filtered

检测方案

99.9%

形式

lyophilized

包装

pkg of 15 mg

技术

cell culture | mammalian: suitable

结合特异性

Peptide Source: Elastin

Peptide Source: Fibrinogen

运输

wet ice

储存温度

−20°C

相关类别

一般描述

PureCol 胶原蛋白为约 97% 的 I 型胶原蛋白,其余由 III 型胶原蛋白组成。 它是可溶性的胶原蛋白。通过凝胶渗透色谱法测得它的高单体含量较高。

它非常适合包被表面和制备薄层用于培养细胞。不建议将 PureCol 冻干形式用于形成固体凝胶。 PureCol 胶原蛋白采用用户友好的包装形式,便于使用和存储。 该产品以无菌冻干粉形式提供。

应用

PureCol胶原蛋白已用于:
  • 制备胶原蛋白凝胶,用于人脐静脉内皮细胞(HUVEC)球状体的三维出芽实验
  • 包被Costar® Transwell®小室培养板,以便进行膀胱平滑肌细胞(BMSC)的细胞迁移实验
  • 包被培养板,以便进行乳腺癌细胞系的胶原降解实验

制备说明

该产品以冻干粉形式提供,含 15 mg 牛胶原蛋白。 用 5 ml 无菌 0.01 N HCl 重悬时,浓度约为 3 mg/ml。

法律信息

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

细胞外基质蛋白质,如层粘连蛋白、胶原蛋白和纤连蛋白,可用作细胞培养中的细胞附着基底。

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3D细胞培养概述。了解2D与3D细胞培养的对比结果,3D细胞培养的优势以及可用于开发3D细胞模型的技术

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