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质量水平
检测方案
≥90.0% (HPLC)
制造商/商品名称
ATTO-TEC GmbH
λ
in acetonitrile: water (1:9) (with 0.01% trifluoroacetic acid)
紫外吸收
λ: 534-540 nm Amax
适用性
suitable for fluorescence
储存温度
−20°C
一般描述
Atto 532是一种新型荧光标记，属于我们熟知的罗丹明6G染料。该染料设计用于生命科学领域，如标记DNA、RNA或蛋白质等。这种标记具有吸收性强、荧光量子产率高、热和光稳定性高、水溶性好、三重态形成非常少等特点。偶联至底物后，Atto 532鬼笔环肽携带-1的净电荷。 鬼笔环肽是从毒性菇类鬼笔鹅膏中分离的真菌毒素。其毒性归因于其在肝脏和肌肉细胞中，与F肌动蛋白结合的能力。由于结合了鬼笔环肽，肌动蛋白丝变得非常稳定。研究人员已经发现鬼笔环肽仅会与肌动蛋白的聚合形式和寡聚形式结合，而不与单体肌动蛋白结合。已确定肌动蛋白-鬼笔环肽复合物的解离常数约为3 x 10-8。 鬼笔环肽在反应速度上不同于鹅膏蕈碱。在高剂量水平下，小鼠或大鼠会在1或2小时内死亡。
应用
鬼笔环肽的罗丹明染色剂类荧光标记物，如鬼笔环肽-Atto 532，可用于标记肌动蛋白丝，从而用于组织学应用中。鬼笔环肽的某些结构特征是与肌动蛋白结合所必需的。但是，氨基酸7（g-二羟亮氨酸）的侧链可进行化学修饰，而对肌动蛋白的亲和力不会发生明显的损失。
法律信息
本产品仅供研究使用。如果打算商业化，请联系知识产权持有者（德国ATTO-TEC GmbH公司）申请许可。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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商品
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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