48275
β 半乳糖苷酶 来源于大肠杆菌
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
别名:
β-D-半乳糖苷半乳糖水解酶, 乳糖酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
~140 U/mg
form
powder
quality
lyophilized
specific activity
~140 U/mg
mol wt
Mr ~135000
contains
phosphate buffer as stabilizer, sucrose as stabilizer
technique(s)
enzyme immunoassay: suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
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General description
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
Application
水溶液可以直接用于衍生化，而无需事先进行透析或凝胶渗透色谱法。
Biochem/physiol Actions
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
Packaging
无底玻璃瓶。内含物装在插入的融合锥内。
Other Notes
1U对应于在pH 7.8和37℃下每分钟水解1 μmol 2-硝基苯基-β-D-吡喃半乳糖苷（目录号73660）所需的酶量。
用于4-甲基伞形酮基-β-D-半乳糖苷（目录号69590）参与的高灵敏度酶联免疫测定人IgG；夹心酶联免疫分析评价β-D-半乳糖苷酶（大肠埃希菌）和辣根过氧化物酶标记物；促甲状腺素释放激素（TRH）的酶联免疫测定；酶-抗体偶联物的制备
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates
K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
M Takeyama et al.
Chemical & pharmaceutical bulletin, 40(8), 2199-2201 (1992-08-01)
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal
S Privitera et al.
The Journal of biological chemistry, 273(11), 6319-6326 (1998-04-16)
Our previous studies showed immunological and functional similarities, as well as partial sequence homology, between the enzymatically inactive alternatively spliced variant of human beta-galactosidase (S-gal) and the 67-kDa elastin/laminin-binding protein (EBP) from sheep. To define the genetic origin of the
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