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46940

Sigma-Aldrich

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯

suitable for fluorescence, ≥75% (HPLC)

别名:

荧光素-5 (6)-氨基己酸 N-琥珀酰亚胺酯

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About This Item

经验公式（希尔记法）:
C31H26N2O10
CAS号:
114616-31-8
分子量:
586.55
MDL编号:
MFCD00467542
UNSPSC代码:
12352108
PubChem化学物质编号:
329757270
NACRES:
NA.32

质量水平

100

方案

≥75% (HPLC)

表单

solid

溶解性

DMF: soluble
methanol: soluble

荧光

λex 490 nm; λem 514 nm (pH 8.2)
λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)C(=O)NCCCCCC(=O)ON6C(=O)CCC6=O)c1.Oc7ccc8c(Oc9cc(O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)NCCCCCC(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C31H26N2O10/c34-18-6-9-22-24(15-18)41-25-16-19(35)7-10-23(25)31(22)21-8-5-17(14-20(21)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37;34-18-6-9-21-24(15-18)41-25-16-19(35)7-10-22(25)31(21)23-14-17(5-8-20(23)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h2*5-10,14-16,34-35H,1-4,11-13H2,(H,32,39)

InChI key

WAGYELYAQOYZFU-UHFFFAOYSA-N

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相关类别

一般描述

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯（FNHS）是一种荧光标签，又称荧光素。FNHS 最大激发波长为 490 nm，最大发射波长为 514 nm。 6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯与伯胺共价结合。

应用

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯（FHS）作为试剂将荧光团6-[荧光素-5(6)-甲酰胺基]己酸偶联到分子上。6-[荧光素-5 (6)-甲酰胺基] 己酸用作基于荧光素的蛋白质荧光标记物，可在猝灭实验等应用中进行分析。
引入荧光素标记的试剂；间隔基使反应性基团更易于生物聚合物上的亲核试剂接触

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315 - H319 - H335

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCH5965
BCCK7257
BCCD3685
BCBX6012
BCBS2220V

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A M Vaz et al.
Colloids and surfaces. B, Biointerfaces, 114, 11-19 (2013-10-29)
In this work, we present a suitable methodology to produce magnetically recoverable bioreactors based on enzymes, which are covalently attached on the surface of iron oxide@silica nanoparticles. In order to produce this system, iron oxide clusters with a mean diameter
A G Johansson et al.
Hepatology (Baltimore, Md.), 24(1), 169-175 (1996-07-01)
Immune complexes were formed between dinitrophenylated human serum albumin (DNP-HSA) and polyclonal rabbit immunoglobulin G (IgG) anti-DNP antibodies at antibody excess. The antigen was labelled with isotope (125I-tyramine-cellobiose) or fluorochrome, (6-[fluorescein-5-(and-6)-carboxamido] hexanoic-acid, succinimidyl ester). The radiolabelled antigen, native or antibody
Avidin binding of carboxyl-substituted biotin and analogues.
K Hofmann et al.
Biochemistry, 21(5), 978-984 (1982-03-02)
J J MacLean et al.
Biophysical journal, 78(3), 1441-1448 (2000-02-29)
Lys-553 of skeletal muscle myosin subfragment 1 (S1) was specifically labeled with the fluorescent probe FHS (6-[fluorescein-5(and 6)-carboxamido]hexanoic acid succinimidyl ester) and fluorescence quenching experiments were carried out to determine the accessibility of this probe at Lys-553 in both the
R Bertrand et al.
Biochemistry, 34(29), 9500-9507 (1995-07-25)
We describe, for the first time, the reaction of skeletal myosin subfragment 1 (S-1) with the succinimido ester of 6-[fluorescein-5(and 6)-carboxamido]hexanoic acid (FHS), which takes place at pH 7.0, 20 degrees C, within a 15 min period, in the presence
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