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Merck
CN
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文件

46940

Sigma-Aldrich

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯

suitable for fluorescence, ≥75% (HPLC)

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别名:
荧光素-5 (6)-氨基己酸 N-琥珀酰亚胺酯
经验公式（希尔记法）:
C31H26N2O10
CAS号:
114616-31-8
分子量:
586.55
MDL编号:
MFCD00467542
UNSPSC代码:
12352108
PubChem化学物质编号:
329757270
NACRES:
NA.32

质量水平

100

检测方案

≥75% (HPLC)

形式

solid

溶解性

DMF: soluble
methanol: soluble

荧光

λex 490 nm; λem 514 nm (pH 8.2)
λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)C(=O)NCCCCCC(=O)ON6C(=O)CCC6=O)c1.Oc7ccc8c(Oc9cc(O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)NCCCCCC(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C31H26N2O10/c34-18-6-9-22-24(15-18)41-25-16-19(35)7-10-23(25)31(22)21-8-5-17(14-20(21)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37;34-18-6-9-21-24(15-18)41-25-16-19(35)7-10-22(25)31(21)23-14-17(5-8-20(23)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h2*5-10,14-16,34-35H,1-4,11-13H2,(H,32,39)

InChI key

WAGYELYAQOYZFU-UHFFFAOYSA-N

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相关类别

一般描述

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯（FNHS）是一种荧光标签，又称荧光素。FNHS 最大激发波长为 490 nm，最大发射波长为 514 nm。 6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯与伯胺共价结合。

应用

6-[荧光素-5 (6)-甲酰胺基] 己酸 N -羟基琥珀酰亚胺酯（FHS）作为试剂将荧光团6-[荧光素-5(6)-甲酰胺基]己酸偶联到分子上。6-[荧光素-5 (6)-甲酰胺基] 己酸用作基于荧光素的蛋白质荧光标记物，可在猝灭实验等应用中进行分析。
引入荧光素标记的试剂；间隔基使反应性基团更易于生物聚合物上的亲核试剂接触

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315 - H319 - H335

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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R Bertrand et al.
Biochemistry, 34(29), 9500-9507 (1995-07-25)
We describe, for the first time, the reaction of skeletal myosin subfragment 1 (S-1) with the succinimido ester of 6-[fluorescein-5(and 6)-carboxamido]hexanoic acid (FHS), which takes place at pH 7.0, 20 degrees C, within a 15 min period, in the presence
A M Vaz et al.
Colloids and surfaces. B, Biointerfaces, 114, 11-19 (2013-10-29)
In this work, we present a suitable methodology to produce magnetically recoverable bioreactors based on enzymes, which are covalently attached on the surface of iron oxide@silica nanoparticles. In order to produce this system, iron oxide clusters with a mean diameter
A G Johansson et al.
Hepatology (Baltimore, Md.), 24(1), 169-175 (1996-07-01)
Immune complexes were formed between dinitrophenylated human serum albumin (DNP-HSA) and polyclonal rabbit immunoglobulin G (IgG) anti-DNP antibodies at antibody excess. The antigen was labelled with isotope (125I-tyramine-cellobiose) or fluorochrome, (6-[fluorescein-5-(and-6)-carboxamido] hexanoic-acid, succinimidyl ester). The radiolabelled antigen, native or antibody
Avidin binding of carboxyl-substituted biotin and analogues.
K Hofmann et al.
Biochemistry, 21(5), 978-984 (1982-03-02)
Abdul Qader Abbady et al.
Frontiers in physiology, 8, 317-317 (2017-06-06)
Programed cell death is a critical and unavoidable part of life. One of the most widely used markers for dying cells, by apoptosis or pyroptosis, is the redistribution of phosphatidylserine (PS) from the inner to the outer plasma membrane leaflet.
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