检测方案
~70%
质量水平
形式
solid
溶解性
H2O: soluble
荧光
λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+
适用性
suitable for fluorescence
储存温度
−20°C
SMILES字符串
Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1
InChI
1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)
InChI key
OZLGRUXZXMRXGP-UHFFFAOYSA-N
应用
Fluo-3和相关分子Fluo3/AM可用作细胞内钙离子（Ca2+）的荧光指示剂。Fluo-3可用于流式细胞术以及使用可见光激发的共焦激光扫描显微术（与在488nm下操作的氩激光源兼容）。与钙结合后，荧光强度增加约40倍。
其他说明
用于流式细胞术的钙离子荧光指示剂。与钙结合后，荧光强度增加约40倍
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
P A Vandenberghe et al.
Journal of immunological methods, 127(2), 197-205 (1990-03-09)
Cytoplasmic free calcium [( Ca2+]i) is a key intracellular messenger in many cell types. We have used fluo-3, a recently developed calcium probe, to study [Ca2+]i in resting and stimulated human peripheral blood T lymphocytes. The spectral properties of fluo-3
Xiao-Ting Huang et al.
Endocrinology, 158(11), 3900-3913 (2017-09-25)
Type 2 diabetes, which features β-cell failure, is caused by the decrease of β-cell mass and insulin secretory function. Current treatments fail to halt the decrease of functional β-cell mass. Strategies to prevent β-cell apoptosis and dysfunction are highly desirable.
Xinjing Guo et al.
Food & function, 10(8), 4661-4673 (2019-07-12)
Hydroxysafflor yellow A (HSYA) is the main active ingredient of edible plant safflower. HSYA has demonstrated anti-inflammatory effects. The inflammatory response is the key mechanism responsible for asthma, and the pro-inflammatory platelet-activating factor (PAF) is known to play a role
C H J Albers-Wolthers et al.
PloS one, 12(6), e0179156-e0179156 (2017-06-27)
Kisspeptins (KPs) and their receptor (GPR54 or KiSS1R) play a key-role in regulation of the hypothalamic-pituitary-gonadal axis and are therefore interesting targets for therapeutic interventions in the field of reproductive endocrinology. As dogs show a rapid and robust LH response
Qijin Zhao et al.
Cell death and differentiation, 26(9), 1600-1614 (2018-11-06)
Mitochondrial superoxide dismutase 2 (SOD2) is a major antioxidant defense enzyme. Here we provide evidence that SOD2 plays critical roles in maintaining calcium homeostasis in newly generated embryonic cerebral cortical neurons, which is essential for normal mitochondrial function and subcellular
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