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Merck
CN

45260

伊红 B

suitable for microscopy

别名:

4′,5′-二溴-2′,7′-二硝基荧光素 二钠盐, Saffrosine, 伊红猩红, 伊红蓝, 酸性红91

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关于此项目

线性分子式:
C20H6N2O9Br2Na2
化学文摘社编号:
分子量:
624.06
EC Number:
208-943-1
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4121964
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SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

technique(s)

titration: suitable

λmax

509-524 nm

suitability

suitable for microscopy

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General description

Eosin B is a fluorescent red dye that is used in microscopy to visualise cytoplasm and proteins like collagen.

Application

Eosin B is an adsorption and fluorescent indicator in microscopy.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

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Xu Wang et al.
Electrophoresis, 31(23-24), 3808-3815 (2010-11-17)
In this study, we describe an effective visualizing technique for proteins in SDS-PAGE based on the organic dye, Eosin B, the sensitivity of which can be further strengthened by the addition of magnesium to the staining solution after electrophoresis. The
Chloé E Atreya et al.
The Journal of biological chemistry, 278(16), 14092-14100 (2003-01-31)
Protozoal parasites are unusual in that their thymidylate synthase (TS) and dihydrofolate reductase (DHFR) enzymes exist on a single polypeptide. In an effort to probe the possibility of substrate channeling between the TS and DHFR active sites and to identify
Jishu Naskar et al.
Organic & biomolecular chemistry, 9(19), 6610-6615 (2011-08-13)
Self-assembling short peptides can offer an opportunity to make useful nano-/microstructures that find potential application in drug delivery. We report here the formation of multivesicular structures from self-assembling water-soluble synthetic amphiphilic dipeptides containing a glutamic acid residue at the C-terminus.
A A Waheed et al.
Analytical biochemistry, 287(1), 73-79 (2000-11-18)
Eosin B and eosin Y have been used to estimate micro- and submicrogram quantities of proteins respectively as shown in our previous reports. In the present study we describe the mechanism of eosin binding to proteins. At pH lower than
Zhong-Xin Zhu et al.
Analytical biochemistry, 426(1), 1-3 (2012-04-10)
A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, stained, and developed within 30 min to achieve transparent and colorless LPS

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