一般描述
含有iTRAQ®试剂113、114、115、116、117、118、119及121，以及足够进行5次8重检测的材料。多重缓冲液试剂盒(货号4381664)需另行订购。
货号4381663产品所含试剂相当于Sciex iChemistry®货号4390812的产品。
货号4381663产品所含试剂相当于Sciex iChemistry®货号4390812的产品。
相对和绝对定量同位素标记(isobaric tags for relative and absolute quantitation)iTRAQ®试剂是首个多重、胺特异性、稳定同位素试剂组合，可同时标记8种不同生物学样本中的所有多肽以进行鉴定及相对和绝对定量，并且保留重要的翻译后修饰(PTM)信息。iTRAQ 8重试剂盒有更经济的大包装可供选择。
应用
iTRAQ® Reagent - 8PLEX Multiplex Kit 8标蛋白质多重检测试剂盒已用于：
- 衍生化和标记碘代乙酰胺探针(IPM)标记的HeLa蛋白组的胰酶消化物
- 相对和绝对定量同位素标记(iTRAQ)多肽
- 作为蛋白组分析的同量异位素(isobar)标记，用于不同消化样本蛋白的比较定量
特点和优势
- 同时对多个样本中的蛋白质进行鉴定和定量
- 标记所有肽段以扩大蛋白和蛋白组覆盖率，包括翻译后修饰(PTM)分子
- 标记每个蛋白的多个肽，提高MS/MS质谱鉴定和定量的可靠性
- 提高结果通量和可靠性，便于进行蛋白质生物标志物研究
- 流程简化，无需进行样品分离以降低样本复杂性，比如蛋白质亲和沉降技术(affinity pull-down)
- 一次实验最多可同时检测8种不同的生物样本
- ProteinPilot软件全面支持所有的SCIEX蛋白组学LC/MS/MS平台
分析说明
法律信息
iChemistry is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
储存分类代码
3 - Flammable liquids
闪点(°F)
35.6 °F - closed cup
闪点(°C)
2.0 °C - closed cup
法规信息
危险化学品
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