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储存温度
−20°C
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一般描述
iTRAQ®试剂是首个多重、胺特异性、稳定同位素试剂组合，可同时标记8种不同生物学样本中的所有多肽以进行鉴定及相对和绝对定量，并且保留重要的翻译后修饰(PTM)信息。
包含iTRAQ® 试剂113、114、115、116、117、118、119及121，以及SDS变性剂、TCEP还原剂、MMTS半胱氨酸封闭试剂、TEAB缓冲液和异丙醇等，可用于最初的实验方案开发。
该试剂盒包含与Sciex iChemistry® 货号4390811相同的试剂。
该试剂盒包含与Sciex iChemistry® 货号4390811相同的试剂。
应用
iTRAQ® Reagent - 8PLEX One Assay Kit蛋白质多重检测试剂盒已用于相对和绝对定量同位素标记(iTRAQ, isobaric tag for relative and absolute quantitation)：
- 芥菜芽总蛋白消化样本
- 西兰花芽总蛋白
- 来源于不同植物/完整叶绿体的脱盐多肽
特点和优势
- 同时对多个样本中的蛋白质进行鉴定和定量
- 标记所有肽段以扩大蛋白和蛋白组覆盖率，包括翻译后修饰(PTM)分子
- 标记每个蛋白的多个肽，提高MS/MS质谱鉴定和定量的可靠性
- 提高结果通量和可靠性，便于进行蛋白质生物标志物研究
- 流程简化，无需进行样品分离以降低样本复杂性，比如蛋白质亲和沉降技术(affinity pull-down)
- 一次实验最多可同时检测8种不同的生物样本
- ProteinPilot软件全面支持所有的SCIEX蛋白组学LC/MS/MS平台
分析说明
法律信息
iChemistry is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°C)
2.0 °C - closed cup
法规信息
危险化学品
常规特殊物品
含少量动物源组分生物产品
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Chao Cheng et al.
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Heat stress has been proved to increase the content of melatonin in plants. In the present study, a combination of methods including physiological and biochemical, gene transcription and proteomic were used to investigate the melatonin accumulation mechanisms in mustard sprouts
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