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文件

41785

溴化二亚胺

Name: 溴化二亚胺
Brand: SIGMA

BioReagent, suitable for fluorescence, ~95% (AT)

别名:

3,8-二氨基-5-甲基-6-苯基菲啶鎓溴化物, 胰蛋白酶, 菲啶盐1553

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About This Item

经验公式（希尔记法）:

C₂₀H₁₈BrN₃

CAS号:

518-67-2

分子量:

380.28

Beilstein:

3809779

EC 号:

208-256-7

MDL编号:

MFCD00011757

UNSPSC代码:

12171500

PubChem化学物质编号:

57650090

NACRES:

NA.32

产品线

BioReagent

质量水平

100

检测方案

~95% (AT)

形式

solid

mp

243-248 °C (lit.)

荧光

λ_ex 306 nm; λ_em 612 nm in 50 mM Tris pH 8.0 (DNA)
λ_ex 480 (pH 7.6, low ionic strength)

适用性

suitable for fluorescence

SMILES字符串

[Br-].C[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C20H17N3.BrH/c1-23-19-12-15(22)8-10-17(19)16-9-7-14(21)11-18(16)20(23)13-5-3-2-4-6-13;/h2-12,22H,21H2,1H3;1H

InChI key

MQOKYEROIFEEBH-UHFFFAOYSA-N

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一般描述

溴甲菲啶是一种抗锥虫药物，已知它可在许多生物体中影响核酸合成，比如组织培养的细胞。

应用

基于碱基对的特异性，溴甲菲啶被用作天然DNA的粘合剂。它在核酸实验中用作荧光剂。

核酸插入探针。

象形图

GHS07

警示用语：

Warning

危险声明

H315,H319,H335

预防措施声明

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

分析证书(COA)

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Nuclear transplantation studies of the action of dimidium bromide on amoebae.

S E Hawkins et al.

Nature, 222(5188), 86-87 (1969-04-05)

A comparison of the base-pair specificities of three phenanthridine drugs using solution spectroscopy.

G Dougherty

The International journal of biochemistry, 14(6), 493-504 (1982-01-01)

1. The absorption spectrum of three phenanthridine drugs (ethidium, dimidium and prothidium bromide) bound to natural DNAs of differing G-C content were obtained using a novel mixing scheme and analysed according to the excluded site binding model. 2. Ethidium bromide

[Chemoprophylaxis of bovine trypanosomiasis T. congolense and T. vivax by phenathridinium S. 1553].

J DEOM

Annales de la Societe belge de medecine tropicale (1920), 30(1), 25-29 (1950-03-01)

[The toxicity of sanguinarine compared to a number of other DNA-tropic compounds for ethidium bromide-sensitive and -resistant transformed murine fibroblasts in culture].

T N Beliaeva et al.

Tsitologiia, 31(11), 1363-1368 (1989-11-01)

A natural DNA-intercalator plant benzo-c-phenanthridine alkaloid sanguinarine is more toxic for mouse transformed fibroblast L-cells in culture than synthetic DNA-intercalator ethidium bromide (EtB) and alkaloid berberine. Dimidium bromide is also an inhibitor of the L-cell growth. In assay conditions, growth

[Effect of some DNA-intercalators and antioxidants on the phagosome- lysosome fusion and F-actin content in murine peritoneal macrophages].

T P Mozhenok et al.

Tsitologiia, 40(6), 585-590 (1998-10-21)

Effect of DNA-intercalators ethidium bromide (EB, 0.005 and 0.015 mM) and dimidium bromide (DB, 0.005 and 0.010 mM) and antioxidative compounds acetylsalicylic acid (ASA, 0.05 and 0.50 mM) and beta-carotene (0.01, 0.02, 0.05 mM) on the phagosome-lysosome (P-L) fusion and

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