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质量水平
方案
≥80.0% (degree of coupling)
分子量
Mw 836.7 g/mol
溶解性
DMF: 0.25 mg/mL, clear
荧光
λex 510 nm; λem 530 nm±5 nm in PBS, pH 7.4
储存温度
−20°C
一般描述
Absorption Maximum, λmax: 517 nm (MeOH),
511 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.24 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.07 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 533 nm (MeOH),
530 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 −620 nm
Fluorescence Quantum Yield, η: 0.82 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 ns (PBS, pH 7.4)
511 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.24 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.07 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 533 nm (MeOH),
530 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 −620 nm
Fluorescence Quantum Yield, η: 0.82 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 ns (PBS, pH 7.4)
应用
Abberior® Star 512 labelled phosphoethanolamine lipid analogues were used for gated STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) study.
适用性
Designed and tested for fluorescent super-resolution microscopy
法律信息
abberior is a registered trademark of Abberior GmbH
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Mathias P Clausen et al.
Methods (San Diego, Calif.), 88, 67-75 (2015-07-01)
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches is the combination of far-field super-resolution stimulated-emission-depletion (STED) microscopy with
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
S W Hell et al.
Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
Beitraege zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung.
Abbe, E.
Archiv fur Mikroskopische Anatomie, 9, 413-420 (1873)
Volker Westphal et al.
Physical review letters, 94(14), 143903-143903 (2005-05-21)
Utilizing single fluorescent molecules as probes, we prove the ability of a far-field microscope to attain spatial resolution down to 16 nm in the focal plane, corresponding to about 1/50 of the employed wavelength. The optical bandwidth expansion by nearly
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