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Merck
CN
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文件

38733

Sigma-Aldrich

Laemmli Lysis缓冲液

non smelling

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UNSPSC代码:
12352200
NACRES:
NA.25

质量水平

100

形式

liquid

组成

bromophenol blue, 0.004%
DTT, 400 mM
glycerol, 20%
SDS, 4%
TRIS, 0.125 M

pH值（酸碱度）

~6.8

适用性

in accordance for electrophoresis test

储存温度

−20°C

相关类别

应用

Laemmli裂解液是一种无气味的裂解缓冲液，可制备用于SDS凝胶电泳的蛋白质样品。

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险声明

H302,H315,H318

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the difference between the two Laemmli buffers S3401 and 38733?

    Product No. S3401 is 0.125 M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125 M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue.  So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).

  4. What is the advantage of using Laemmli Lysis-buffer, Product No. 38733?

    The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol).  Some people are very sensitive to the odor of 2-mercaptoethanol.

  5. Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?

    For most applications, it is considered to be a 2X solution.

  6. Why do the Laemmli sample buffers like S3401 and 38733 have glycerol in them?

    An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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