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Merck
CN

38371

Sigma-Aldrich

Atto 488 蛋白标记试剂盒

BioReagent, suitable for fluorescence

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

产品线

BioReagent

质量水平

制造商/商品名称

ATTO-TEC GmbH

荧光

λex 488 nm; λem 520 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

适用性

suitable for fluorescence

储存温度

2-8°C

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一般描述

该试剂盒包含反应性染料、缓冲液和蛋白纯化套件,足够用于进行五个标记反应(每个反应1 mg蛋白)并对标记的蛋白进行纯化。

应用

Atto 荧光标记设计用于高灵敏度应用,包括单分子检测。Atto 标记具有刚性结构,未显示任何顺反异构化。因此,这些标记物在偶联时显示出极低的光谱偏移。Atto 488 蛋白标记试剂盒可用于通过偶联伯胺基团生成Atto 488标记的蛋白。

法律信息

本产品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Sheng Shu et al.
Nature communications, 13(1), 4576-4576 (2022-08-06)
Lipopolysaccharide (LPS) is an essential glycolipid and forms a protective permeability barrier for most Gram-negative bacteria. In E. coli, LPS levels are under feedback control, achieved by FtsH-mediated degradation of LpxC, which catalyzes the first committed step in LPS synthesis.
Andrea Pallares Pallares et al.
Food & function, 9(12), 6544-6554 (2018-11-28)
The presence of cell walls entrapping starch granules in common bean cotyledons, prevailing after thermal processing and mechanical disintegration, has been identified as the main reason for their (s)low in vitro starch digestibility. Nevertheless, it is unknown if the role
Switching modulation for protein labeling with activatable fluorescent probes.
Kalyan K Sadhu et al.
Chembiochem : a European journal of chemical biology, 12(9), 1299-1308 (2011-06-02)
Daniela C Dieterich
Current opinion in neurobiology, 20(5), 623-630 (2010-07-24)
Fluorescent proteins have revolutionized cell biology and, therefore, our understanding of the complex molecular and cellular mechanisms that wire the brain together and enable its plasticity throughout life. The ability to visualize cell biological processes has inspired the development of
Gražvydas Lukinavičius et al.
Current opinion in chemical biology, 15(6), 768-774 (2011-11-15)
Numerous synthetic fluorophores have been developed that can switch their spectroscopic properties upon interaction with other molecules or by irradiation with light. In recent years, protein-labeling techniques have been introduced that permit the specific attachment of such molecules to proteins

商品

Protein labeling kits with Atto and Tracy dyes provide easy fluorescent labeling of purified proteins, enzymes, and antibodies.

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