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Merck
CN

38132

Sigma-Aldrich

N-(5-荧光素基)马来酰亚胺

≥90% (HPLC), BioReagent, suitable for fluorescence

别名:

5-马来酰亚胺荧光素

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About This Item

经验公式(希尔记法):
C24H13NO7
CAS号:
分子量:
427.36
MDL编号:
UNSPSC代码:
12352111
PubChem化学物质编号:
NACRES:
NA.32

产品线

BioReagent

质量水平

方案

≥90% (HPLC)

荧光

λex 490 nm; λem 518 nm in 0.1 M Tris pH 8.0

适用性

corresponds for coupling to thiols
suitable for fluorescence

储存温度

2-8°C

SMILES字符串

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1

InChI

1S/C24H13NO7/c26-13-2-5-17-19(10-13)31-20-11-14(27)3-6-18(20)24(17)16-4-1-12(9-15(16)23(30)32-24)25-21(28)7-8-22(25)29/h1-11,26-27H

InChI key

AYDAHOIUHVUJHQ-UHFFFAOYSA-N

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一般描述

N-(5-荧光素基)马来酰亚胺,又称荧光素-5-马来酰亚胺(FM),是一种荧光衍生化试剂。在衍生化反应过程中,pH值起着关键作用。

应用

N-(5-荧光素基)马来酰亚胺或荧光素-5-马来酰亚胺可用作荧光生物传感器检测纳米颗粒。

N-(5-荧光素基)马来酰亚胺(5-FM)可用于通过其硫醇基团对分子进行荧光标记,例如蛋白质和肽。

包装

无底玻璃瓶。内含物在插入的融合锥体内。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Acute Tox. 4 Oral

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

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Oluyemi Akinloye et al.
Analytical and bioanalytical chemistry, 409(4), 903-915 (2016-12-03)
The ability to detect and quantify nanoparticles is essential but there is currently no simple, sensitive, and rapid method for the detection of nanomaterials. We have developed a novel peptide-based fluorescence-based biosensor for detection and measurement of negatively charged engineered
Michael Raba et al.
Journal of molecular biology, 382(4), 884-893 (2008-08-12)
Selected residues of transmembrane domain (TM) IX were previously shown to play key roles in ligand binding and transport in members of the Na(+)/solute symporter family. Using the Na(+)/proline transporter PutP as a model, a complete Cys scanning mutagenesis of
Nicola Giangregorio et al.
Biochimica et biophysica acta, 1767(11), 1331-1339 (2007-10-27)
During substrate translocation mitochondrial carriers cycle between the cytoplasmic-state (c-state) with substrate-binding site open to the intermembrane space and matrix-state (m-state) with the binding site open to the mitochondrial matrix. Here, the accessibility of Cys-58, Cys-136 and Cys-155 of the
Chuck R Smallwood et al.
Molecular microbiology, 72(5), 1171-1180 (2009-05-13)
We studied the reactivity of 35 genetically engineered Cys sulphydryl groups at different locations in Escherichia coli FepA. Modification of surface loop residues by fluorescein maleimide (FM) was strongly temperature-dependent in vivo, whereas reactivity at other sites was much less
Sattasuk Kwanchanok et al.
Bioscience, biotechnology, and biochemistry, 75(10), 2001-2007 (2011-10-08)
Despite recent progress in fluorescence techniques employed to observe protein localization in living cells, the in vitro chloroplastic protein transport assay remains a useful tool for determining the destinations of proteins. Although an in vitro synthesized, radiolabeled precursor protein is

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