biological source
rabbit
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
EP103, monoclonal
description
For In Vitro Diagnostic Use in Select Regions
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (371R-24)
vial of 0.1 mL concentrate Research Use Only (371R-24-RUO)
vial of 0.5 mL concentrate (371R-25)
vial of 1.0 mL concentrate (371R-26)
vial of 1.0 mL concentrate Research Use Only (371R-26-RUO)
vial of 1.0 mL pre-dilute Research Use Only (371R-27-RUO)
vial of 1.0 mL pre-dilute ready-to-use (371R-27)
vial of 7.0 mL pre-dilute ready-to-use (371R-28)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (371R-28-RUO)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200 (concentrated)
isotype
IgG
control
squamous carcinoma (lung)
shipped in
wet ice
storage temp.
2-8°C
visualization
nuclear
Gene Information
human ... SOX2(6657)
Analysis Note
 IVD |  IVD |  IVD |  RUO |
General description
The differentiation of seminomas from non-seminomatous germ cell tumors can be challenging, especially, if small biopsy specimens, necrotic tumors and metastatic tumors with artifacts are encountered. A subset of germ cell tumors may require immunohistochemistry (IHC) for classification owing to unusual morphologic features, such as diffuse growth of clear cells, and tumors with glandular and/or microcytic patterns. In the mixed germ cell tumor, one component is often intermingled intimately with others such as embryonal carcinoma versus yolk sac tumor, can be overlooked. IHC will identify such an area and allow for the identification of each component of the mixed tumor more accurately and documenting them in the pathology report is recommended by WHO. Current IHC studies have shown the combination of CD30/CD117 staining plays a good role in distinguishing between embryonal carcinoma and yolk sac tumor. However, a subset of tumors may not be distinguished by this combination. Also, the characteristic membranous pattern by antibodies to CD30 and CD117 for the interpretation of the diagnosis may not be evident in limited biopsy specimens. In this respect, transcription factors, such as SOX-2, are easier to interpret due to their distinct nuclear reaction. SOX-2 has been reported as a diagnostic marker for embryonal carcinoma. SOX-2 was expressed in intratubular embryonal carcinoma, pure embryonal carcinoma and in the embryonal carcinoma component of mixed germ cell tumor in all cases. But, SOX-2 expression has not been found in seminoma, yolk sac tumor, and choriocarcinoma in almost all cases. An inclusion of transcription factors, like SOX-2 and SOX-17, in a panel of IHC is useful for the classification of germ cell tumors: that is, SOX-2+/OCT3/4+/CD30+/CD117-/SOX17- for embryonal carcinoma; SOX-2-/OCT3/4+/CD30-/CD117+/SOX17+ for seminoma; SOX-2-/OCT3/4-/CD30-/CD117-/SOX17- for yolk sac tumor.
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
SOX-2 Positive Control Slides, Product No. 371S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.
Preparation Note
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Note: This IFU may not apply to your specific product lot.
Note: This requires a keycode which can be found on your packaging or product label.
Download the latest released IFU
Note: This IFU may not apply to your specific product lot.
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
J de Jong et al.
The Journal of pathology, 215(1), 21-30 (2008-03-19)
Combined action of SOX and POU families of transcription factors plays major roles in embryonic development. In embryonic stem cells, the combination of SOX2 and POU5F1 (OCT3/4) is essential for maintaining the undifferentiated state by activating pluripotency-linked genes, and inhibition
Tumors of the testis and paratesticular tissue
Woodward PJ et al.
Pathology and Genetics of Tumours of the Urinary System and Male Genital Organs, Pages 217-Pages 278 (2004)
I A Sesterhenn et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 10(1), 69-78 (1992-01-01)
The Testicular Cancer Intergroup Study (TCIS) was undertaken to evaluate the pathologic findings in early-stage testicular cancer as determined by central pathology review, to compare these findings with the interpretation by the contributing pathologists, and to make correlations with various
Sandro Santagata et al.
The American journal of surgical pathology, 31(6), 836-845 (2007-05-29)
The core embryonic stem cell transcription factors (TFs) OCT3/4 (OCT4), NANOG, and SOX2 have shared as well as nonoverlapping roles in stem cell growth and differentiation. These same TFs are also expressed in various types of human germ cell tumors
Robert E Emerson et al.
Seminars in diagnostic pathology, 22(1), 33-50 (2006-03-04)
Although most testicular and paratesticular tumors can be recognized by their light microscopic features, some raise significant differential diagnostic questions. Immunohistochemical staining has proved of significant value in this situation. There is still a role for the traditional markers, including
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