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35848

Sigma-Aldrich

2′,7′-二氯荧光素

BioReagent, suitable for fluorescence, ≥90% (T)

别名:

DCF, 二氯荧光黄

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About This Item

经验公式（希尔记法）:
C20H10Cl2O5
CAS号:
76-54-0
分子量:
401.20
Beilstein:
58009
EC 号:
200-968-6
MDL编号:
MFCD00005047
UNSPSC代码:
12171500
PubChem化学物质编号:
57649439
NACRES:
NA.32

产品线

BioReagent

质量水平

100

方案

≥90% (T)

表单

solid

mp

280 °C (dec.) (lit.)

荧光

λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0

适用性

suitable for fluorescence

SMILES字符串

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

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相关类别

一般描述

2′,7′-二氯荧光素，也称为 DCF，是一种荧光染料。DCF 荧光激发波长为 498nm，发射波长为 522nm。 亲脂性非荧光 Dcfh-DA 通过被动扩散和脱乙酰作用很容易穿过细胞膜。脱酰产物是一种对氧化剂敏感的 2′,7′-二氯荧光素（DCHF）。 DCHF 随后被氧化以形成高荧光 DCF，这是被测量的。

应用

2′,7′-二氯荧光素用作荧光探针，用于测量 ROS（活性氧），用于生物学研究。 它也可作为预浸剂，用于荧光测定邻位二醇、糖苷和酚的预浸剂。

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储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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分析证书(COA)

Lot/Batch Number
BCCM5883
BCCL6807
BCCJ9431
BCCG9996
BCCF6570

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What can be used to make a solution of 2´,7´-dichlorofluorescein?

    2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.

  6. In what types of applications can 2´,7´-Dichlorofluorescein be used?

    Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.  See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a  fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).

  7. What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?

    2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

W.D. Pfeffer et al.
Journal of Chromatography A, 506, 401-401 (1990)
H. Watanabe et al.
Analytical Sciences, 2, 461-461 (1986)
Masahito Miura et al.
Journal of molecular and cellular cardiology, 56, 97-105 (2012-12-26)
Ca(2+) waves are initiated not only by Ca(2+) leak from the sarcoplasmic reticulum (SR), but also by Ca(2+) dissociation from the myofilaments in the myocardium with nonuniform contraction. We investigated whether contractile properties and the production of reactive oxygen species
Xiuping Chen et al.
Free radical research, 44(6), 587-604 (2010-04-08)
Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However
C Rota et al.
Free radical biology & medicine, 27(7-8), 873-881 (1999-10-09)
The oxidation of 2'-7'-dichlorofluorescin (DCFH) to the fluorescent 2'-7'-dichlorofluorescein (DCF) by horseradish peroxidase (HRP) was investigated by fluorescence, absorption, and electron spin resonance spectroscopy (ESR). As has been previously reported, HRP/H2O2 oxidized DCFH to the highly fluorescent DCF. However, DCF
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