biological source
rabbit
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
EPR3158Y, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (328R-14)
vial of 0.5 mL concentrate (328R-15)
bottle of 1.0 mL predilute (328R-17)
vial of 1.0 mL concentrate (328R-16)
bottle of 7.0 mL predilute (328R-18)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
isotype
IgG
control
tonsil
shipped in
wet ice
storage temp.
2-8°C
visualization
nuclear
Gene Information
human ... SPI1(6688)
Analysis Note
 IVD |  IVD |  IVD |  RUO |
General description
PU.1 is a transcription factor that has been shown to be important for normal B-cell development. PU.1 belongs to the ETS family of transcription factors. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. Very little is known about PU.1 function in later stages of B-cell development. PU.1 does not seem to play a role in the end-stage of B-cell development and is not expressed in plasma cells. PU.1 exerts an important role in the regulation of the expression of crucial B-cell proteins, such as immunoglobulin (Ig) genes, and CD20 and its putative binding sites were also identified in the promoters of CD79, CD10, and CD22. PU.1 binds to the 3′ enhancer region of both the Ig kappa and lambda light chain genes and it also regulates the immunoglobulin heavy chain genes through the intron enhancer region. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker including the following: B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma.
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
PU.1 Positive Control Slides, Product No. 328S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
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R Hromas et al.
Blood, 82(10), 2998-3004 (1993-11-15)
The ETS oncogene family member PU.1 is a transcriptional activator that is dysregulated by Friend erythroleukemia virus insertion. Northern analysis found that PU.1 is highly expressed in cells of myeloid and B-lymphoid origin, but not expressed at all in a
Christoph Loddenkemper et al.
The Journal of pathology, 202(1), 60-69 (2003-12-25)
It has previously been demonstrated that in cultured and in situ tumour cells of classical Hodgkin lymphoma (cHL), the immunoglobulin (Ig) promoter is inactive and its transcription factors Oct2 and/or BOB.1/OBF.1 are down-regulated. In this study, the analysis of these
Juliette J Hoefnagel et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 19(9), 1270-1276 (2006-06-17)
Expression patterns of eight transcription factors involved in different stages of B-cell development were investigated in a large group of primary cutaneous B-cell lymphomas and compared with expression patterns during normal B-cell development. The following transcription factors were investigated: Pax-5
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