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主要文件

安全信息

3023

Sigma-Aldrich

AptaFluor® SAH甲基转移酶测定

别名:

甲基转移酶活性检测

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0.4 ML
CN¥1,578.88

CN¥1,578.88


预计发货时间May 20, 2025详情


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选择尺寸

变更视图
0.4 ML
CN¥1,578.88

About This Item

UNSPSC代码:
41122100
NACRES:
NA.84

CN¥1,578.88


预计发货时间May 20, 2025详情


获取大包装报价

运输

dry ice

储存温度

−70°C

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此商品
706237062470920
technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

shipped in

wet ice

shipped in

dry ice, wet ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

一般描述

AptaFluor® SAH甲基转移酶TR-FRET分析是将S-腺苷甲硫氨酸(SAM)转化为S-腺苷同型半胱氨酸(SAH)的酶的通用分析方法,具有远红色的时间分辨Förster共振能量转移(TR-FRET)读数。该测定法可用于组蛋白甲基转移酶(HMT)和DNA甲基转移酶(DNMT)家族中的所有酶。该测定法专为高通量筛选(HTS)设计,具有简单的混合和读取格式和2个液体添加步骤。它在终点模式下运行:用通用终止试剂(提供)淬灭甲基转移酶(MT)酶反应,然后加入检测试剂。

该测定试剂具有试剂稳定性,并与常用的多模式酶标仪兼容。AptaFluor® SAH甲基转移酶TR-FRET分析具有以下优点:
  • 简单,混合和读取格式非常适合HTS。
  • 在初始速度条件下(=SAM到SAH的20%转换)进行稳健的MT检测。
  • 可容纳低至100 nM和高至5 μM的SAM浓度。
  • 远红色示踪剂进一步减少了荧光化合物和光散射的干扰。

查看完整的Transcreener®产品列表

数量

3023-B=100次测定,96孔
3023-1K=1,000次测定,384孔

外形

含缓冲水溶液的试剂盒

法律信息

AptaFluor is a registered trademark of BellBrook Labs
Transcreener is a registered trademark of BellBrook Labs

法规信息

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