Transcreener^® UDP² TR-FRET Assay

The Transcreener^® UDP2 TR-FRET Red Assay is a competitive immunoassay for UDP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for UDP, the assay can be used with any enzyme that generates UDP from a UDP-sugar donor. Examples include glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetylgalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose, and hyaluronan synthases. The Transcreener^® assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener^® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener^® UDP2 TR-FRET Red Assay provides the following benefits:

• Accommodates UDP-sugar donor concentrations ranging from 1 μM to 1,000 μM.
• Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10–30%).
• Overcomes the need for time-consuming, one-off assay development for individual members within an enzyme family by using a single set of assay reagents that detect an invariant product.
• Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
• Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener^® product list

3022-1K = 1,000 assay, 384-well
3022-10K = 10,000 assay, 384-well

Kit with buffered aqueous solutions

Transcreener is a registered trademark of BellBrook Labs