推荐产品
运输
dry ice
储存温度
−20°C
一般描述
The Transcreener® GDP TR-FRET Red Assay is a competitive immunoassay for GDP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for GDP, the assay can be used with any enzyme that converts GTP to GDP, regardless of what other substrates are used. Examples of enzymes include GTPases and fucosyltransferases. The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.
The Transcreener® GDP TR-FRET Red Assay provides the following benefits:
View full Transcreener® product list
The Transcreener® GDP TR-FRET Red Assay provides the following benefits:
- Accommodates GTP concentrations ranging from 0.1 μM to 1,000 μM.
- Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10–30%).
- Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
- Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
- Far-red tracer further minimizes interference from fluorescent compounds and light scattering.
View full Transcreener® product list
数量
3021-1K = 1,000 assay, 384-well
3021-10K = 10,000 assay, 384-well
3021-10K = 10,000 assay, 384-well
外形
Kit with buffered aqueous solutions
法律信息
Transcreener is a registered trademark of BellBrook Labs
法规信息
常规特殊物品
Cord blood in vitro expanded CD41 cells: identification of novel components of megakaryocytopoiesis.
Journal of thrombosis and haemostasis : JTH, 4(4), 848-860 (2006-04-26)
Megakaryopoiesis represents a multi-step, often unclear, process leading to commitment, differentiation, and maturation of megakaryocytes (MKs) that release platelets. To identify the novel genes that might help to clarify the molecular mechanisms of megakaryocytopoiesis and be regarded as potential candidates
Human reproduction (Oxford, England), 20(12), 3469-3475 (2005-08-27)
The pathway of spermatogenesis involves the conversion of diploid stem cells (spermatogonia) to tetraploid primary spermatocytes, followed by meiosis and two cell divisions, first forming diploid secondary spermatocytes and then haploid round spermatids. Differentiation of round spermatids results in spermatozoa
Journal of the American Society of Nephrology : JASN, 32(2), 357-374 (2021-01-01)
Injury to kidney podocytes often results in chronic glomerular disease and consecutive nephron malfunction. For most glomerular diseases, targeted therapies are lacking. Thus, it is important to identify novel signaling pathways contributing to glomerular disease. Neurotrophic tyrosine kinase receptor 3
Nucleic acids research, 46(6), 3019-3033 (2018-03-09)
Organized in tandem repeat arrays in most eukaryotes and transcribed by RNA polymerase III, expression of 5S rRNA genes is under epigenetic control. To unveil mechanisms of transcriptional regulation, we obtained here in depth sequence information on 5S rRNA genes
Investigative ophthalmology & visual science, 61(5), 47-47 (2020-05-27)
To clarify the expression profiles of ion channels and transporters of metabolic substrates among heterogeneous cultured human corneal endothelial cells (cHCECs) distinct in their effectiveness in reconstituting the corneal endothelium. Integrated proteomics for cell lysates by liquid chromatography-tandem mass spectrometry
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门