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Merck
CN
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主要文件

安全信息

3020

Sigma-Aldrich

Transcreener® AMP2/GMP2 TR-FRET Assay

别名:

TR-FRET assay, Transcreener assay

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选择尺寸

W326305-SAMPLE
¥657.65
100 G
¥1,004.39
1 KG
¥2,224.48
5 KG
¥9,259.99

¥657.65


国内现货,预计发货时间2025年4月07日详情



选择尺寸

变更视图
W326305-SAMPLE
¥657.65
100 G
¥1,004.39
1 KG
¥2,224.48
5 KG
¥9,259.99

About This Item

UNSPSC代码:
41122100
NACRES:
NA.84

¥657.65


国内现货,预计发货时间2025年4月07日详情


运输

dry ice

储存温度

−20°C

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751200763500723029
storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

average Mn 8,000

mol wt

-

mol wt

-

mol wt

-

mp

88-92 °C

mp

-

mp

42-47 °C

mp

-

form

powder

form

solid

form

solid

form

solid

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

100

一般描述

The Transcreener® AMP2/GMP2 TR-FRET Red Assay is a competitive immunoassay for enzymes that produce AMP or GMP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The assay relies on a highly specific monoclonal antibody that recognizes AMP or GMP with more than 1,000-fold selectivity over substrate nucleotides, including ATP, cAMP, or cGMP. Enzymes that can be used with the assay include ubiquitin, SUMO, nucleic acid and protein ligases, phosphodiesterases (PDEs), and synthetases. The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® AMP2/GMP2 TR-FRET Red Assay provides the following benefits:
  • Accommodates substrate concentrations ranging from 0.1 μM to 1,000 μM.
  • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10–30%).
  • The only assay method that allows detection of unmodified AMP and GMP without using coupling enzymes.
  • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
  • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener® product list

数量

3020-1K = 1,000 assay, 384-well
3020-10K = 10,000 assay, 384-well

外形

Kit with buffered aqueous solutions

法律信息

Transcreener is a registered trademark of BellBrook Labs

法规信息

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