产品名称
Atto 550-Biotin, BioReagent, suitable for fluorescence, ≥90% (HPLC)
product line
BioReagent
assay
≥90% (HPLC)
form
film
manufacturer/tradename
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 553-559 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550-Biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Immunohistochemical methods for the demonstration of tumor markers.
H Denk
Current topics in pathology. Ergebnisse der Pathologie, 77, 47-69 (1987-01-01)
J Oertel et al.
Journal of cancer research and clinical oncology, 126(8), 425-440 (2000-08-29)
Immunocytochemical investigations of fixed cells are used to enhance diagnostic accuracy in haematology and oncology. The alkaline-phosphatase/anti(alkaline phosphatase) technique, immunoperoxidase and the avidin-biotin technique are the most important methods in immunocytochemistry. Tyramide-enhanced immunostaining is a new powerful technique. Peripheral blood
J Chevalier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(4), 481-491 (1997-04-01)
Biotin was recently applied to detect cellular DNA or RNA. In combination with avidin, streptavidin or antibody, it can be conjugated with fluorescent dye, enzyme, ferritin, or gold. However, emphasis has recently been placed on the false-positive results that are
Robert H Meltzer et al.
Lab on a chip, 11(5), 863-873 (2011-01-21)
Rapid, specific, and sensitive detection of airborne bacteria, viruses, and toxins is critical for biodefense, yet the diverse nature of the threats poses a challenge for integrated surveillance, as each class of pathogens typically requires different detection strategies. Here, we
商品
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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