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Merck
CN

28562

Sigma-Aldrich

Atto 488 马来酰亚胺

BioReagent, suitable for fluorescence, ≥90% (HPLC)

别名:

Atto 488

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About This Item

MDL编号:
UNSPSC代码:
12352111
NACRES:
NA.32

产品线

BioReagent

质量水平

检测方案

≥90% (HPLC)
≥90% (degree of coupling )

制造商/商品名称

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

紫外吸收

λ: 501-506 nm Amax

适用性

suitable for fluorescence

储存温度

−20°C

一般描述

Atto 488马来酰亚胺是一种亲水的荧光标记,具有高分子吸收(90.000)和量子产率(0.80)。在最大501纳米下可激发其荧光活性,523纳米下达到最大发射率。 Atto 488马来酰亚胺针对氩激光器的激发进行了优化,并具有高光稳定性。马来酰亚胺水解形成同分异构的无活性马来酸混合物的反应在硫醇修饰后可以大体完成,在pH高于8的条件下更是如此。偶联至底物后,该标记物携带-1的净电荷。

应用

Atto 488马来酰亚胺用于标记胺,其要求的pH值高于马来酰亚胺和硫醇反应的pH值。它非常适合硫醇基团的荧光标记。

法律信息

本产品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
Muller, P., et al. et al.
Current Microscopy Contributions to Advances in Science and Technology, 1, 3-12 (2012)
Mohd A Mohd Ridzuan et al.
PloS one, 7(3), e33845-e33845 (2012-04-06)
An actomyosin motor complex assembled below the parasite's plasma membrane drives erythrocyte invasion by Plasmodium falciparum merozoites. The complex is comprised of several proteins including myosin (MyoA), myosin tail domain interacting protein (MTIP) and glideosome associated proteins (GAP) 45 and
Sonal et al.
Journal of cell science, 132(4) (2018-12-13)
Dynamic reorganization of the actomyosin cytoskeleton allows fast modulation of the cell surface, which is vital for many cellular functions. Myosin-II motors generate the forces required for this remodeling by imparting contractility to actin networks. However, myosin-II activity might also
Jeanne C Stachowiak et al.
Nature cell biology, 14(9), 944-949 (2012-08-21)
Curved membranes are an essential feature of dynamic cellular structures, including endocytic pits, filopodia protrusions and most organelles. It has been proposed that specialized proteins induce curvature by binding to membranes through two primary mechanisms: membrane scaffolding by curved proteins
E Pourkarimi et al.
Cell death and differentiation, 19(3), 406-415 (2011-09-03)
In C. elegans, the BH3-only domain protein EGL-1, the Apaf-1 homolog CED-4 and the CED-3 caspase are required for apoptosis induction, whereas the Bcl-2 homolog CED-9 prevents apoptosis. Mammalian B-cell lymphoma 2 (Bcl-2) inhibits apoptosis by preventing the release of

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