25455
4-氯-7-硝基苯并-2-氧杂-1,3-二唑
BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
别名:
4-氯-7-硝基-1,2,3-苯并噁二唑, NBD-chloride
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关于此项目
经验公式（希尔记法）:
C6H2ClN3O3
化学文摘社编号:
分子量:
199.55
UNSPSC Code:
12352100
NACRES:
NA.32
PubChem Substance ID:
EC Number:
233-496-4
Beilstein/REAXYS Number:
614212
MDL number:
Assay:
≥97.0% (HPLC)
InChI key
IGHBXJSNZCFXNK-UHFFFAOYSA-N
InChI
1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
SMILES string
[O-][N+](=O)c1ccc(Cl)c2nonc12
product line
BioReagent
assay
≥97.0% (HPLC)
mp
97-100 °C, 97-99 °C (lit.)
fluorescence
λex 336 nm in methanol, λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)
suitability
suitable for fluorescence
Quality Level
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Application
4-氯-7-硝基苯并-2-氧杂-1,3-二唑（NBD-chloride）是一种荧光检测试剂，用于标记蛋白质中的游离巯基和 N-末端。NBD-chloride 可用作酶抑制剂，以及半胱氨酸硫酸的捕获剂。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Leslie B Poole et al.
Methods in enzymology, 348, 122-136 (2002-03-12)
C165S AhpC in its sulfenate (Cys-SO-) and presumed thiolate (Cys-S-) forms at pH 7 (pKa for sulfenic acid about pH 6.1) exhibit low extinction absorbance bands around 367 and 324 nm, respectively. Sulfenic acid content of the protein can be
Lina F Bernal-Perez et al.
Analytical biochemistry, 428(1), 13-15 (2012-06-09)
A fluorogenic derivatization method was developed to distinguish the protein N-terminal acetylation status. The unacetylated protein selectively reacted with 4-chloro-7-nitrobenzofurazan (NBD-Cl) at neutral pH to provide high fluorescence. In contrast, the protein with N-terminal acetylation was essentially nonfluorescent under the
Abhinav Kanwal et al.
Analytical biochemistry, 429(1), 70-75 (2012-07-17)
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Marcus A Kramer et al.
Small (Weinheim an der Bergstrasse, Germany), 8(24), 3791-3794 (2012-08-18)
P D Bragg et al.
Biochimica et biophysica acta, 1413(3), 159-171 (1999-11-11)
Pyridine nucleotide transhydrogenases of bacterial cytosolic membranes and mitochondrial inner membranes are proton pumps in which hydride transfer between NADP(+) and NAD(+) is coupled to proton translocation across cytosolic or mitochondrial membranes. The pyridine nucleotide transhydrogenase of Escherichia coli is
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