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Merck
CN

25455

Sigma-Aldrich

4-氯-7-硝基苯并-2-氧杂-1,3-二唑

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

别名:

4-氯-7-硝基-1,2,3-苯并噁二唑, NBD-chloride

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About This Item

经验公式(希尔记法):
C6H2ClN3O3
CAS号:
分子量:
199.55
Beilstein:
614212
EC 号:
MDL编号:
UNSPSC代码:
12352100
PubChem化学物质编号:
NACRES:
NA.32

产品线

BioReagent

质量水平

方案

≥97.0% (HPLC)

mp

97-100 °C
97-99 °C (lit.)

荧光

λex 336 nm in methanol
λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)

适用性

suitable for fluorescence

SMILES字符串

[O-][N+](=O)c1ccc(Cl)c2nonc12

InChI

1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChI key

IGHBXJSNZCFXNK-UHFFFAOYSA-N

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应用

4-氯-7-硝基苯并-2-氧杂-1,3-二唑(NBD-chloride)是一种荧光检测试剂,用于标记蛋白质中的游离巯基和 N-末端。NBD-chloride 可用作酶抑制剂,以及半胱氨酸硫酸的捕获剂。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


历史批次信息供参考:

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Leslie B Poole et al.
Methods in enzymology, 348, 122-136 (2002-03-12)
C165S AhpC in its sulfenate (Cys-SO-) and presumed thiolate (Cys-S-) forms at pH 7 (pKa for sulfenic acid about pH 6.1) exhibit low extinction absorbance bands around 367 and 324 nm, respectively. Sulfenic acid content of the protein can be
Lina F Bernal-Perez et al.
Analytical biochemistry, 428(1), 13-15 (2012-06-09)
A fluorogenic derivatization method was developed to distinguish the protein N-terminal acetylation status. The unacetylated protein selectively reacted with 4-chloro-7-nitrobenzofurazan (NBD-Cl) at neutral pH to provide high fluorescence. In contrast, the protein with N-terminal acetylation was essentially nonfluorescent under the
Abhinav Kanwal et al.
Analytical biochemistry, 429(1), 70-75 (2012-07-17)
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Parallel dip-pen nanolithography using spore- and colloid-terminated cantilevers.
Marcus A Kramer et al.
Small (Weinheim an der Bergstrasse, Germany), 8(24), 3791-3794 (2012-08-18)
P D Bragg et al.
Biochimica et biophysica acta, 1413(3), 159-171 (1999-11-11)
Pyridine nucleotide transhydrogenases of bacterial cytosolic membranes and mitochondrial inner membranes are proton pumps in which hydride transfer between NADP(+) and NAD(+) is coupled to proton translocation across cytosolic or mitochondrial membranes. The pyridine nucleotide transhydrogenase of Escherichia coli is

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