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安全信息

23479

Sigma-Aldrich

硝酸盐/亚硝酸盐检测试剂盒（比色法）

used for detection of nitric oxide metabolites

别名:

硝酸盐/亚硝酸盐检测试剂盒（比色法）

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

应用

一氧化氮（NO）是一种气态的顺磁性原子团，是生物系统中一种非常重要且通用的信使分子。NO可通过NO合成酶（NOS）由L-精氨酸合成得到它可作为内皮细胞舒张因子（EDRF）及抗血小板药物。它还可作为噬中性粒细胞产生的神经递质及已活化的巨噬细胞产生的细胞毒素。虽然NO在生物系统的分子功能是多种多样的，不过NO最重要的功能是激活鸟苷酸环化酶。

Griess分析方法是一种用来检测NO浓度的最常用及最简单的方法。Griess分析的原理是汇总重氮化合物间的含氮耦合，这种耦合是来由磺胺与NO2及萘二胺形成的。硝酸盐2/亚硝酸盐2 检测试剂盒含有多种染料，一种硝酸还原酶，一种酶辅因子，缓冲溶液及作为标准品的NO2和NO3溶液。因此，使用该试剂盒可以轻松地对总NO代谢产物进行检测。适用的NO2 检测范围是10到100μM。

仅试剂盒组分

产品编号
说明
  • NaNO2 Standard Solution (100 mM) 1 bottle
  • NaNO3 Standard Solution (100 mM) 1 bottle
  • Buffer solution (20 mM, pH 7.6) 1 bottle
  • Nitrate reductase (lyophilized) 1 bottle
  • Enzyme Co-factor (lyophilized) 1 bottle
  • Griess Reagent A 1 bottle
  • Griess Reagent B 1 bottle

相关产品

产品编号
说明
价格

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Warning

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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分析证书(COA)

Lot/Batch Number
BCCL9064
BCCL3034
BCCK3359
BCCK7778
BCCK2381

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Fredrik Sjövall et al.
PloS one, 9(5), e97673-e97673 (2014-05-16)
The levels of nitric oxide (NO) and various cytokines are known to be increased during sepsis. These signaling molecules could potentially act as regulators and underlie the enhancement of mitochondrial function described in the later phase of sepsis. Therefore, we
Mohamed Raâfet Ben Khedher et al.
Lipids in health and disease, 16(1), 241-241 (2017-12-14)
Vasculogenic erectile dysfunction (VED) is considered as a common complication among people with type 2 diabetes (T2D). We tested whether changes in fatty acid (FAs) classes measured in erythrocytes are associated with increased risk of diabetic VED along with related
Othman A Al-Shboul et al.
Journal of pregnancy, 2019, 4302309-4302309 (2019-05-14)
It is well known that pregnancy is associated with frequent gastrointestinal (GI) disorders and symptoms. Moreover, previous reports have shown that estrogen, which changes in levels during pregnancy, participates in the regulation of GI motility and is involved in the
Jolanta Zuwała-Jagiełło et al.
Clinical and experimental hepatology, 1(3), 105-111 (2015-11-01)
To determine plausible associations between liver cirrhosis and circulating endothelial cell-derived microparticles (EMPs), vascular endothelial growth factor (VEGF) levels and plasma nitric oxide (NO) metabolites. Sixty patients with cirrhosis and 20 healthy control subjects were enrolled in the study. Circulating
Othman A Al-Shboul et al.
Biomedical reports, 9(6), 511-516 (2018-12-14)
Previous studies have shown that progesterone could inhibit muscle contraction in various sites of the gastrointestinal tract. The underlying mechanisms responsible for these inhibitory effects of progesterone are not fully known. The aim of the current study was to investigate
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