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产品线
BioReagent
质量水平
方案
>90.0% (HPLC)
表单
powder
制造商/商品名称
ATTO-TEC GmbH
透射比
254 nm
565 nm
荧光
λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
λ
(ethanol with 0.1% trifluoroacetic acid)
紫外吸收
λ: 560-566 nm Amax
适用性
corresponds for coupling to thiols
suitable for fluorescence
储存温度
−20°C
应用
Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
法律信息
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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