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Merck
CN

18507

Sigma-Aldrich

Atto 565 maleimide

BioReagent, suitable for fluorescence

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About This Item

经验公式(希尔记法):
C37H37ClN4O10
分子量:
733.16
MDL编号:
UNSPSC代码:
12352111
NACRES:
NA.32

产品线

BioReagent

质量水平

方案

>90.0% (HPLC)

表单

powder

制造商/商品名称

ATTO-TEC GmbH

透射比

254 nm
565 nm

荧光

λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0

λ

(ethanol with 0.1% trifluoroacetic acid)

紫外吸收

λ: 560-566 nm Amax

适用性

corresponds for coupling to thiols
suitable for fluorescence

储存温度

−20°C

应用

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

法律信息

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

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