16805
Atto 425 NHS酯
BioReagent, suitable for fluorescence, ≥90% (HPLC)
别名:
Atto 425-N-羟基琥珀酰亚胺酯
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关于此项目
经验公式（希尔记法）:
C26H30N2O8
化学文摘社编号:
分子量:
498.53
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352108
MDL number:
产品名称
Atto 425 NHS酯, BioReagent, suitable for fluorescence, ≥90% (HPLC)
Quality Level
InChI
1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3
SMILES string
CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)ON4C(=O)CCC4=O)c3cc2OC1=O
InChI key
PUEQEMDTFPYCDY-UHFFFAOYSA-N
product line
BioReagent
assay
≥90% (HPLC)
≥90% (degree of coupling)
manufacturer/tradename
ATTO-TEC GmbH
solubility
DMF: soluble
DMSO: soluble
H2O: soluble
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 422-428 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
Application
Atto 425 NHS 酯用于开发双功能荧光无机微粒（Zeolite L 晶体），设计用于靶向或成像多组分。与氨基或胺修饰的寡核苷酸反应形成稳定的胺键。用作分子信标的基本组份，旨在用于实时 PCR 分析，以快速、准确地鉴定革兰氏阴性细菌。当与苄基鸟嘌呤偶联时，Atto 425-NHS 的荧光被淬灭，但在标记 SNAP 标签融合蛋白后变为荧光，这使该偶联物成为活细胞实验的理想候选者。在三色 FRET 实验中用作供体探针之一，旨在研究 RNA-蛋白质复合物形成的动力学。
Atto 荧光标签专门为高灵敏度应用而设计，包括单分子检测。Atto 标签具有刚性结构，不显示任何顺式-反式-异构化。因此，这些标签具有超高强度，并且在共轭时具有最小的光谱偏移。
General description
Atto 425 是市售香豆素衍生物的成员。
Legal Information
本产品仅供研究使用。如果打算商业化，请联系知识产权持有者（德国ATTO-TEC GmbH公司）申请许可。
Other Notes
在荧光相关光谱法（FCS）中研究一光子激发和两光子激发的稳定性；用于三色重合分析。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Handbook of Biophotonics: Vol. 2: Photonics for Health Care (2011)
P.S. Dittrich, P. Schwille
Applied Physics B: Lasers and Optics, 73, 829-829 (2001)
Katrin G Heinze et al.
Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of
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