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一般描述
多聚-D-赖氨酸（PDL）和层粘连蛋白溶液非常适合用于细胞培养表面的涂层方案。PDL层粘连蛋白涂层广泛用于神经培养。已知它在体外细胞培养过程中可以改善细胞粘附和细胞成熟。多聚-D-赖氨酸和层粘连蛋白溶液是在磷酸盐缓冲生理盐水中进行制备并经无菌过滤。它用于涂覆组织培养器皿以达到0.25 ml/cm2的培养物表面比。组织培养器皿在室温下涂覆一小时并小心吸出溶液。在以PBS洗涤两次后，涂覆的组织培养器皿可立即使用或风干后置于4oC保存最长一周。
应用
多聚-D-赖氨酸&层粘连蛋白（2.5 ML）已用作体外诱导剂，刺激 Y79 细胞表达神经元和神经胶质细胞特征。它已用于涂布视网膜神经节细胞培养的培养板。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
J Tombran-Tink et al.
Investigative ophthalmology & visual science, 30(8), 1700-1707 (1989-08-01)
Tumor cells can be induced to differentiate in vitro by biochemical manipulation of their culture environment. In the studies described here, the effects of medium conditioned by human retinal pigmented epithelial (RPE) cells on Y79 human retinoblastoma cells have been
A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro
Aurelie Stil, et al.
Frontiers in Cellular Neuroscience (2023)
The protective role of sulforaphane and Homer1a in retinal ischemia-reperfusion injury: Unraveling the neuroprotective interplay
Saadh MJ, et al.
Basic Life Sciences (2023)
A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro
Stil A, et al.
Frontiers in Cellular Neuroscience, 17, 1212097-1212097 (2023)
Jing-Wen Yu et al.
Journal of molecular neuroscience : MN, 60(4), 486-497 (2016-08-31)
Bone marrow-derived mesenchymal stem cells (MSCs) are the ideal transplanted cells of cellular therapy for promoting neuroprotection and neurorestoration. However, the optimization of transplanted cells and the improvement of microenvironment around implanted cells are still two critical challenges for enhancing
实验方案
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。
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