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生物来源
bovine
描述
protease substrate
技术
electrophoresis: suitable
溶解性
water: 5 mg/mL
ε (消光系数)
32-38 at 440 nm in 0.1 M NaOH at 1% (1 cm)
储存温度
2-8°C
一般描述
蛋白水解活性测定的显色底物；酶消化导致形成有色组分，该组分可溶于三氯乙酸并且可在366-450nm范围内测定（经过中和后）。
应用
适合作为蛋白水解酶的底物，可用于蛋白酶的电泳后检测。
生化/生理作用
在淀粉样蛋白加工研究中，可用于诱导小鼠继发性淀粉样变性。
其他说明
销售限制可能适用
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
D Vázquez Peyronel et al.
Electrophoresis, 16(10), 1894-1897 (1995-10-01)
Azocasein, diffused into gels after polyacrylamide gel electrophoresis, provides a useful substrate for simple and rapid protease detection. After electrophoresis, the gels are incubated in an azocasein solution, immersed in trichloroacetic acid and then treated with NaOH. Proteolytic zones appear
Pineapple cysteine endopeptidases.
A D Rowan et al.
Methods in enzymology, 244, 555-568 (1994-01-01)
Fanqi Wang et al.
Immunology, 159(1), 109-120 (2019-10-14)
Serpins are evolutionarily conserved serine protease inhibitors that are widely distributed in animals, plants and microbes. In this study, we reported the cloning and functional characterizations of two novel serpin genes, HlSerpin-a and HlSerpin-b, from the hard tick Haemaphysalis longicornis
Shih-Hua Lo et al.
Animal bioscience, 35(10), 1592-1605 (2022-04-26)
The objective of this study was to select an effective in vitro digestion-fermentation model to estimate the effect of decreasing dietary crude protein (CP) on odor emission during pig production and to suggest potential prediction markers through in vitro and
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