product name
2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸) 二铵盐, ≥98% (HPLC)
质量水平
检测方案
≥98% (HPLC)
形式
powder or crystals
杂质
≤6.0% water
溶解性
H2O: 10 mg/mL, slightly hazy to clear
储存温度
2-8°C
SMILES字符串
[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
InChI
1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key
OHDRQQURAXLVGJ-AXMZSLBLSA-N
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应用
2,2′-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）联胺盐可用于：
- 通过自由基清除法测试赤松树皮（PDB）提取物的抗氧化特性
- 测定芦荟样本的抗氧化性（AOC）
- 在酶联免疫吸附测定（ELISA）中用作链霉亲和素-辣根过氧化物酶（HRPO）的底物
2,2′-联氮基-双3-乙基苯并噻唑啉-6-磺酸)是一种过氧化物酶底物，适用于ELISA程序。该底物产生一种可溶性最终产物，其颜色为绿色，可在405nm分光光度法下读取。用1%十二烷基硫酸钠(SDS)可使反应停止。 推荐用于ELISA (微孔)程序，不推荐用于膜应用。
其他说明
过氧化物酶的显色底物 ；EIA 中的 ABTS-H2O2-过氧化物酶系统
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
A Szutowicz et al.
Analytical biochemistry, 138(1), 86-94 (1984-04-01)
Monoamine oxidase is assayed in tissue by a colorimetric reaction using horse radish peroxidase and 2,2'-azinodi(ethylbenzthiazoline-6-sulfonic acid to measure H2O2 formed during oxidation of amines. The method has a coefficient of variation of approximately 2.5% and provides results comparable with
Antioxidant and cytotoxicological effects of Aloe vera food supplements
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The tubers of Bletilla formosana were fermented with eight plant pathogen fungi, respectively, and antioxidant activities and total phenolic content (TPC) of the crude extracts of fermented products and non-fermented products were investigated. The antioxidant activities were evaluated in three
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Enzyme immunoassay ELISA and EMIT.
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