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生物来源
human esophagus
质量水平
形式
liquid
生长模式
Adherent
核型
Modal number 59 chromosomal aberration at 11p13-15.
形态学
Epithelial growing in islands
产品
Expression of actin imtermediate filaments and growth factors TGF?1, TGF?2, TGF?3, TGF? and platelet-derived growth factor A (PDGFA). P53 mutation c.298C>T
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Oesophagus adenocarcinoma, well-differentiated
应用
For complete product information, please see ECACC
Recommended culture medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
培养基
RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cell population doubling time 39 hours.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections supplied by us, are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
E Rodriguez et al.
Cancer research, 50(19), 6410-6416 (1990-10-01)
In a cytogenetic analysis of 9 gastric and lower esophageal adenocarcinomas, we detected nonrandom rearrangements involving the region 11p13-15 in 8, thus identifying for the first time a specific chromosomal lesion in these tumors. In addition, rearrangements involving 3p21, translocations
N Altorki et al.
Cancer, 72(3), 649-657 (1993-08-01)
Gastric carcinoma is one of the most common malignancies worldwide, with an overall survival of about 10%. Improvement in therapy awaits better understanding of the biologic behavior of this tumor. Establishment of cell lines permits detailed analysis of the biology
G K Schwartz et al.
Journal of the National Cancer Institute, 85(5), 402-407 (1993-03-03)
Gastric adenocarcinoma is a common neoplasm worldwide. Patients with completely resected disease often have locoregional recurrence, and adjuvant chemotherapy has failed to reduce the common occurrence of metastases. Protein kinase C (PKC) is thought to be important in tumor cell
Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the
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