推荐产品
生物来源
bovine lung
质量水平
检测方案
~80% (HPCE)
形式
crystalline (fine)
质量
lyophilized
比活
≥3500 U/mg
分子量
~6,500
技术
electrophoresis: suitable
颜色
white
溶解性
phosphate buffer: 5 mg/mL, clear, colorless (PH 7.6 (70 mm phosphate))
UniProt登记号
储存温度
2-8°C
InChI key
ZPNFWUPYTFPOJU-UHFFFAOYSA-N
基因信息
cow ... PTI(404172)
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应用
抑肽酶被大量用作胰蛋白酶抑制剂。
生化/生理作用
抑肽酶是一种竞争性丝氨酸蛋白酶抑制剂，可与酶的活性位点组成稳定的复合酶并同时对其进行封闭。 这种结合是可逆的，而且绝大多数抑肽酶-蛋白酶复合物可在极端的pH >10 or < 3水平时发生解离。 从结构上来说，抑肽酶是一种来自牛肺的单体球状蛋白，由58个氨基酸组成并排列在具有三个交联二硫键的单个多肽链中。
单位定义
一个胰蛋白酶抑制剂单位（TIU）可将两个胰蛋白酶单位的活性降低50％，而其中一个胰蛋白酶单位在pH 7.8和25℃下可每分钟水解1.0 μ摩尔的N-α-苯甲酰基-DL-精氨酸对硝基苯胺。另一种常用的单位是KIU，而1 TIU = 1,300 KIU。
1 U corresponds to 1 biological kallikrein inhibitor unit (KIU)
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
Monica Averna et al.
Methods in molecular biology (Clifton, N.J.), 1915, 195-206 (2019-01-09)
Immunoblotting is a procedure routinely used to analyze calpastatin expression. However, immunoblotting alone may not be adequate for this task, since calpastatin isoforms can vary by tissue, can be modified by partial digestion, and can undergo posttranslational modifications. Here we
Hamid Ebrahimi Orimi et al.
Scientific reports, 10(1), 9730-9730 (2020-06-18)
We introduced and validated a drop-on-demand method to print cells. The method uses low energy nanosecond laser (wavelength: 532 nm) pulses to generate a transient microbubble at the distal end of a glass microcapillary supplied with bio-ink. Microbubble expansion results in
Roberta De Tullio et al.
Methods in molecular biology (Clifton, N.J.), 1915, 163-185 (2019-01-09)
The production of recombinant calpastatin in E. coli has become an efficient tool to obtain discrete amounts of a specific calpastatin species that can be present concomitantly with other calpastatin fragments/forms in the same tissue or cell type in a
Axel Himmelbach et al.
Bio-protocol, 8(15), e2955-e2955 (2018-08-05)
Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution and throughput of chromosome conformation capture
Elizabeth J Osterlund et al.
Science advances, 8(16), eabm7375-eabm7375 (2022-04-21)
Cytoplasmic and membrane-bound BCL-2 family proteins regulate apoptosis, a form of programmed cell death, via dozens of binary protein interactions confounding measurement of the effects of inhibitors in live cells. In cancer, apoptosis is frequently dysregulated, and cell survival depends
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