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Merck
CN

10419

Sigma-Aldrich

8-苯胺基-1-萘磺酸 半镁盐 水合物

for fluorescence, ≥95.0% (T)

别名:

8-苯胺-1-萘磺酸镁 水合物, ANSA 镁盐, N-苯基-1-萘胺-8-磺酸 镁盐, 苯基-1-萘胺-8-磺酸 镁盐

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About This Item

经验公式(希尔记法):
C16H12Mg0.5NO3S · xH2O
CAS号:
分子量:
310.49 (anhydrous basis)
Beilstein:
4834623
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

等级

for fluorescence

质量水平

检测方案

≥95.0% (T)

形式

powder

溶解性

hot water: 50 mg/mL, green

λmax

465-475 nm

荧光

λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

O.[Mg++].[O-]S(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12.[O-]S(=O)(=O)c4cccc5cccc(Nc6ccccc6)c45

InChI

1S/2C16H13NO3S.Mg.H2O/c2*18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;;/h2*1-11,17H,(H,18,19,20);;1H2/q;;+2;/p-2

InChI key

FBANPIUXAXTAFX-UHFFFAOYSA-L

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一般描述

ANSA (8-Anilino-1-naphthalensulfonic acid) 是一种疏水性极性敏感的荧光染料,可作为位点探针用于检测细胞和胶束膜以及蛋白质等分子的构象变化。ANSA 也用作荧光探针,用于估算表面活性剂的临界胶束浓度 (CMC)。

特点和优势

用于估算表面活性剂CMC的荧光探针。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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M Bertsch et al.
Analytical biochemistry, 313(2), 187-195 (2003-02-28)
Hydrophobic sites on the surface of protein molecules are thought to have important functional roles. The identification of such sites can provide information about the function and mode of interaction with other cellular components. While the fluorescence enhancement of polarity-sensitive
Rapid fluorescent "staining" of nondenatured protein bands in agar and polyacrylamide gels.
S T Nerenberg et al.
Analytical biochemistry, 43(2), 564-574 (1971-10-01)
J L Jensen et al.
Cellular and molecular biology (Noisy-le-Grand, France), 46(3), 685-696 (2000-06-29)
We have studied the metal-catalyzed oxidation (MCO) of brain-derived neurotrophic factor (BDNF) with regard to target sites and potential conformational changes of the protein. The exposure of BDNF to three different levels of ascorbate/Cu(II)/O2 [20 microM Cu(II), 2 mM ascorbate
C D Peters et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 75(1), 55-61 (1997-01-01)
Protein aggregation is believed to be due to conformers that expose hydrophobic clusters that promote protein association. Such conformers can be detected using a fluorescent probe like 8-anilino 1-naphthalenesulfonic acid (ANSA). Here we show that urease exposed to 1.0 M
Anilinonaphthalene sulfonate as a probe of membrane composition and function.
J Slavík
Biochimica et biophysica acta, 694(1), 1-25 (1982-08-11)

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