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Merck
CN

10065

Sigma-Aldrich

α-淀粉酶 来源于米曲霉

powder, ~30 U/mg

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别名:
1,4-α-D-葡聚糖-葡萄糖水解酶
EC 号:
MDL编号:
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

形式

powder

质量水平

比活

~30 U/mg

分子量

Mr ~51000

储存温度

2-8°C

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一般描述

微生物淀粉酶是胞外酶,可用于多种工业应用,如面包和麦芽糖浆生产、酱油和豆面酱发酵等。从米曲霉中分离的α-淀粉酶的分子量为51kDa(沉降和扩散)和49kDa(凝胶过滤)。

应用

α-淀粉酶已被用作
  • 琼脂平板和羧甲基纤维素清除试验的对照酶,用于筛选淀粉水解的纤维素酶活性,
  • 以便探讨小麦淀粉在冷冻面团中
  • 抑制金黄色葡萄球菌生物膜的作用。

生化/生理作用

米曲霉α-淀粉酶(Ao α-淀粉酶)酶催化可溶性淀粉和相关底物中α-1,4糖苷键的水解。这些底物被分解并释放较短的低聚糖和α-极限糊精。

单位定义

1个酶活力单位(U)相当于在pH6.0和25℃下每分钟释放1μmol麦芽糖所需的酶量(淀粉符合Zulkowsky,货号85642,作为底物)。

其他说明

用于测定食物中的脂肪;应用于糖苷键的(选择性)水解/缩合

象形图

Health hazardExclamation mark

警示用语:

Danger

危险声明

危险分类

Acute Tox. 4 Oral - Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

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Han Tao et al.
Food chemistry, 265, 18-22 (2018-06-10)
Properties of starches isolated from soft and hard wheat dough after freezing/thawing (F/T) treatment were investigated. Significance of results was observed between isolated hard wheat starch (HWS) and soft wheat starch (SWS), but both cultivars showed an increase in the
Han Tao et al.
Food chemistry, 190, 588-593 (2015-07-28)
The wheat dough was subjected to freezing/thawing treatment for 0, 3, 7, and 10 cycles and fractionated into non-gluten proteins and starch. High-performance liquid chromatography revealed changes in molecular mass distribution occurred for the extracted non-gluten proteins. As for the
K. Fujita et al.
Bulletin of the Chemical Society of Japan, 62, 3150-3150 (1989)
Mamadou Abdoulaye Konaré et al.
Plants (Basel, Switzerland), 12(2) (2023-01-22)
Currently, microencapsulation has become a viable method of nutrient delivery for the food industry. This work microencapsulated the bioactive compounds extracted from two neglected species (Balanites aegyptiaca and Ziziphus mauritiana) by freeze-drying. A combination of wall materials (whey protein and
S Wirsel et al.
Molecular microbiology, 3(1), 3-14 (1989-01-01)
We have cloned three genes (amy1, amy2 and amy3) encoding alpha-amylase in the filamentous fungus Aspergillus oryzae. The established overall sequences have a very high degree of homology, showing divergences mainly in the 3'-untranslated regions. The positions and the sequences

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