08951
6-Carboxy-fluorescein diacetate N-succinimidyl ester
≥90.0% (HPLC)
别名:
6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester
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关于此项目
经验公式（希尔记法）:
C29H19NO11
化学文摘社编号:
分子量:
557.46
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352108
MDL number:
产品名称
6-Carboxy-fluorescein diacetate N-succinimidyl ester, ≥90.0% (HPLC)
InChI
1S/C29H19NO11/c1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(=O)ON6C(=O)CCC6=O)c1
InChI key
JGPOSNWWINVNFV-UHFFFAOYSA-N
assay
≥90.0% (HPLC)
solubility
DMF: soluble
DMSO: soluble
fluorescence
λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
suitability
suitable for fluorescence
storage temp.
−20°C
Application
The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells.
Other Notes
Reagent for continuously determining the intracellular pH in bacteria by fluorometry; labeling human intervertebral disk cells in vitro for fluorescence microscopy
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
H E Gruber et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 75(3), 118-123 (2000-08-19)
We have assessed the utility of an intracellular fluorochrome, 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), as a tracking label for human intervertebral disc cells in vitro. Although 5 microM provides adequate intracellular labeling for whole cell fluorescent microscopic identification of labeled
P Breeuwer et al.
Applied and environmental microbiology, 62(1), 178-183 (1996-01-01)
A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria. cFSE can be taken up by bacteria in the form of its diacetate
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