biological source
human lymph
form
liquid
growth mode
Non-adherent or adherent on poly-L-lysine treated plastic
karyotype
2n=46
morphology
Lymphoblast
products
Adult T-cell leukaemia virus (ALT; HTLV-1)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
-65 to -96°C
Biochem/physiol Actions
人T细胞白血病
未指定
来源于一名患有全身性淋巴结肿大和肝脾肿大（成人T细胞白血病）的45岁日本女性，通过将其外周血白细胞与男性脐带血淋巴细胞共培养。这些细胞携带有HTLV-1并支持HIV的生长。细胞应在实验室密闭等级3条件下处理。MT-2具有男性的核型，并在ECACC上通过STR分析证实了Y染色体的存在
Preparation Note
RPMI 1640 + 2 mM 谷氨酰胺 + 10% 去补体胎牛血清 (FBS)。
按2-3x100,000细胞/ml进行铺板培养。饱和密度为9 x 100,000细胞/ml；5% CO2；37 °C.
Other Notes
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
S Harada et al.
Science (New York, N.Y.), 229(4713), 563-566 (1985-08-09)
The human T-cell lines MT-2 and MT-4 carry the human T-cell leukemia virus type I (HTLV-I). When MT-2 and MT-4 were infected with HTLV-III, the probable etiologic agent of the acquired immune deficiency syndrome (AIDS), rapid cytopathogenic effects and cytotoxicity
S Harada et al.
Virology, 146(2), 272-281 (1985-10-30)
Early events in the infection of the human T-lymphotropic virus type-I (HTLV-I)-positive MT-4 cell line by the acquired immune deficiency syndrome (AIDS) retrovirus HTLV-III were investigated. The virus was adsorbed completely to the cells within 60 min incubation after inoculation
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