产品名称
Atto 532, BioReagent, suitable for fluorescence, ≥90% (HPCE)
product line
BioReagent
assay
≥90% (HPCE)
manufacturer/tradename
ATTO-TEC GmbH
fluorescence
λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
Application
Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性，构型不会产生任何顺反异构变化。因此这种标记偶联后的强度表现卓越，光谱转变却又极低。
Atto 532已与二抗偶联，用于STED（刺激发射耗竭）显微镜和免疫荧光研究。
Atto 532已与二抗偶联，用于STED（刺激发射耗竭）显微镜和免疫荧光研究。
General description
Atto 532是一种新的标签，具有高分子吸收（115,000）和量子产率（0.90），并且在激发和发射最大值之间具有足够的斯托克斯′位移。它针对倍频Nd:YAG激光器的激发进行了优化，并具有高光稳定性。
Legal Information
本产品仅供研究使用。如果打算商业化，请联系知识产权持有者（德国ATTO-TEC GmbH公司）申请许可。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
3D reconstruction of high-resolution STED microscope images.
Punge A et al.
Microscopy Research and Technique, 71, 644-644 (2008)
Uffe V Schneider et al.
BMC biotechnology, 10, 4-4 (2010-01-28)
Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV
Evidence for major structural changes in subunit C of the vacuolar ATPase due to nucleotide binding.
Andrea Armbrüster et al.
FEBS letters, 579(9), 1961-1967 (2005-03-29)
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit
STED microscopy reveals nanoparticle assemblies
Willig, K.I., et al.
New Journal of Physics, 8, 1-1 (2006)
Chunlai Chen et al.
Nucleic acids research, 35(9), 2875-2884 (2007-04-14)
Hybridization of nucleic acids with secondary structure is involved in many biological processes and technological applications. To gain more insight into its mechanism, we have investigated the kinetics of DNA hybridization/denaturation via fluorescence resonance energy transfer (FRET) on perfectly matched
商品
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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