产品线
BioReagent
质量水平
检测方案
≥90% (HPCE)
制造商/商品名称
ATTO-TEC GmbH
荧光
λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0
适用性
suitable for fluorescence
储存温度
−20°C
一般描述
Atto 532是一种新的标签，具有高分子吸收（115,000）和量子产率（0.90），并且在激发和发射最大值之间具有足够的斯托克斯′位移。它针对倍频Nd:YAG激光器的激发进行了优化，并具有高光稳定性。
应用
Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性，构型不会产生任何顺反异构变化。因此这种标记偶联后的强度表现卓越，光谱转变却又极低。
Atto 532已与二抗偶联，用于STED（刺激发射耗竭）显微镜和免疫荧光研究。
Atto 532已与二抗偶联，用于STED（刺激发射耗竭）显微镜和免疫荧光研究。
法律信息
本产品仅供研究使用。如果打算商业化，请联系知识产权持有者（德国ATTO-TEC GmbH公司）申请许可。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Uffe V Schneider et al.
BMC biotechnology, 10, 4-4 (2010-01-28)
Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV
3D reconstruction of high-resolution STED microscope images.
Punge A et al.
Microscopy Research and Technique, 71, 644-644 (2008)
Kiyoto Kamagata et al.
Journal of the American Chemical Society, 134(28), 11525-11532 (2012-06-14)
A method was developed to detect fluorescence intensity signals from single molecules diffusing freely in a capillary cell. A unique optical system based on a spherical mirror was designed to enable quantitative detection of the fluorescence intensity. Furthermore, "flow-and-stop" control
Moritz Marcinowski et al.
Nature structural & molecular biology, 18(2), 150-158 (2011-01-11)
The endoplasmic reticulum is the site of folding, assembly and quality control for proteins of the secretory pathway. The ATP-regulated Hsp70 chaperone BiP (heavy chain-binding protein), together with cochaperones, has important roles in all of these processes. The functional cycle
Chunlai Chen et al.
Nucleic acids research, 35(9), 2875-2884 (2007-04-14)
Hybridization of nucleic acids with secondary structure is involved in many biological processes and technological applications. To gain more insight into its mechanism, we have investigated the kinetics of DNA hybridization/denaturation via fluorescence resonance energy transfer (FRET) on perfectly matched
商品
荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
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