05019
7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin
BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
别名:
MDCC
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关于此项目
经验公式（希尔记法）:
C20H21N3O5
化学文摘社编号:
分子量:
383.40
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.32
MDL number:
InChI key
IXQPRUQVJIJUEB-UHFFFAOYSA-N
InChI
1S/C20H21N3O5/c1-3-22(4-2)14-6-5-13-11-15(20(27)28-16(13)12-14)19(26)21-9-10-23-17(24)7-8-18(23)25/h5-8,11-12H,3-4,9-10H2,1-2H3,(H,21,26)
product line
BioReagent
assay
≥97.0% (HPLC)
suitability
suitable for fluorescence
Quality Level
Application
7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin is utilized as a fluoresencent biological sensing device . Used for real-time measurements for the release of inorganic phosphates during enzymatic reaction when MDCC is conjugated to a mutant phosphate-binding protein . Also, utilized for intramolecular fluorescence energy transfer (FRET) experiments .
Thiol-reactive probe for protein labelling
M Brune et al.
Biochemistry, 33(27), 8262-8271 (1994-07-12)
A probe has been developed that can rapidly measure micromolar concentrations of inorganic phosphate (Pi), in particular to follow the release of Pi in real time from enzymes such as phosphatases. Its application is described to investigate the mechanism of
Yu-Chih Tsai et al.
Biochemistry, 45(32), 9675-9687 (2006-08-09)
We show that T7 DNA polymerase exists in three distinct structural states, as reported by a conformationally sensitive fluorophore attached to the recognition (fingers) domain. The conformational change induced by a correct nucleotide commits the substrate to the forward reaction
Z H He et al.
The Journal of physiology, 501 ( Pt 1), 125-148 (1997-05-15)
1. The rate of appearance of inorganic phosphate (Pi) and hence the ATPase activity of rabbit psoas muscle in single permeabilized muscle fibres initially in rigor was measured following laser flash photolysis of the P3-1-(2-nitrophenyl)ethyl ester of ATP (NPE-caged ATP)
A Vandecandelaere et al.
Biochemistry, 38(25), 8179-8188 (1999-07-01)
The molecular mechanism underlying microtubule dynamic instability depends on the relationship between the addition of tubulin-GTP to a growing microtubule and its hydrolysis in the microtubule lattice to tubulin-GDP, with release of inorganic phosphate (Pi). Since this relationship remains controversial
Z He et al.
Biophysical journal, 75(5), 2389-2401 (1998-10-28)
Inorganic phosphate (Pi) release was determined by means of a fluorescent Pi-probe in single permeabilized rabbit soleus and psoas muscle fibers. Measurements of Pi release followed photoliberation of approximately 1.5 mM ATP by flash photolysis of NPE-caged ATP in the
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