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一般描述
腈水解酶属于C-N水解酶超家族。它具有催化三联体基序：谷氨酸，赖氨酸和半胱氨酸，分布在真菌，植物和动物中。该蛋白的等电点（pI）为8.1。天然聚丙烯酰胺凝胶电泳（PAGE）。
应用
腈水解酶可用于丙烯酰基交联纤维素二醛（ACCD）的固定化，用于催化活性研究。
生化/生理作用
腈水解酶催化氨的释放和腈形成羧酸。腈水解酶的寡聚结构组织对其功能至关重要。它显示出广泛的底物特异性。
包装
无底玻璃瓶。内含物装在插入的融合锥内。
单位定义
1U对应于在pH 7.2和25℃下每分钟释放1 μmol氨且丙烯腈转化为丙烯酸的酶的量
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Ahmed Kamal et al.
Journal of microbiology and biotechnology, 21(1), 37-42 (2011-02-09)
A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was
Joanna E Raczynska et al.
Journal of structural biology, 173(2), 294-302 (2010-11-26)
The nitrilase superfamily is a large and diverse superfamily of enzymes that catalyse the cleavage of various types of carbon-nitrogen bonds using a Cys-Glu-Lys catalytic triad. Thermoactive nitrilase from Pyrococcus abyssi (PaNit) hydrolyses small aliphatic nitriles like fumaro- and malononitryl.
Shivani Jamwal et al.
International journal of biological macromolecules, 131, 117-126 (2019-03-08)
Immobilization of enzymes to improve their catalytic properties is an attractive protocol which makes them suitable candidates to meet various industrial demands. Present study describes the synthesis of new acryloyl crosslinked cellulose dialdehyde (ACCD) for nitrilase immobilization. Nitrilase was immobilized
Jeremy D Woodward et al.
Communications biology, 1, 186-186 (2018-11-13)
Nitrilases are oligomeric, helix-forming enzymes from plants, fungi and bacteria that are involved in the metabolism of various natural and artificial nitriles. These biotechnologically important enzymes are often specific for certain substrates, but directed attempts at modifying their substrate specificities
Alena Petříčková et al.
Applied microbiology and biotechnology, 93(4), 1553-1561 (2011-09-06)
Nitrilases from Aspergillus niger CBS 513.88, A. niger K10, Gibberella moniliformis, Neurospora crassa OR74A, and Penicillium marneffei ATCC 18224 were expressed in Escherichia coli BL21-Gold (DE3) after IPTG induction. N. crassa nitrilase exhibited the highest yield of 69,000 U L(-1)
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