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Merck
CN

03004

Sigma-Aldrich

Abberior® STAR 512, maleimide

for STED application

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UNSPSC代码:
12352111
NACRES:
NA.32

质量水平

分子量

Mw 861.7 g/mol

浓度

≥50% (degree of coupling)

溶解性

DMF: 0.25 mg/mL, clear

荧光

λex 512 nm; λem 530 nm±5 nm in PBS, pH 7.4

储存温度

−20°C

一般描述

Absorption Maximum, λmax: 517 nm (MeOH),
511 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.24 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.07 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 533 nm (MeOH),
530 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 −620 nm
Fluorescence Quantum Yield, η: 0.82 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 ns (PBS, pH 7.4)

应用

Abberior® Star 512 labelled phosphoethanolamine lipid analogues were used for gated STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) study.

适用性

Designed and tested for fluorescent super-resolution microscopy

法律信息

abberior is a registered trademark of Abberior GmbH

相关产品

产品编号
说明
价格

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Mathias P Clausen et al.
Methods (San Diego, Calif.), 88, 67-75 (2015-07-01)
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches is the combination of far-field super-resolution stimulated-emission-depletion (STED) microscopy with
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
S W Hell et al.
Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation

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