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Merck
CN

01810

Sigma-Aldrich

环己酰亚胺,大包装

≥90% (HPLC)

别名:

3- [2-(3,5-二甲基-2-氧代环己基)-2-羟乙基]戊二酰亚胺, 放线菌酮, 纳拉霉素A

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About This Item

经验公式(希尔记法):
C15H23NO4
CAS号:
分子量:
281.35
Beilstein:
88868
EC 号:
MDL编号:
UNSPSC代码:
51101500
PubChem化学物质编号:
NACRES:
NA.85

生物来源

microbial

质量水平

检测方案

≥90% (HPLC)

形式

powder or crystals

颜色

white to light beige

抗生素抗菌谱

fungi
yeast

作用机制

protein synthesis | interferes

InChI

1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1

InChI key

YPHMISFOHDHNIV-FSZOTQKASA-N

基因信息

相关类别

应用

环十二碳三烯(CHX)被广泛用于抗CHX的酵母和真菌菌株的选择、蛋白质合成的受控抑制以检测短寿命蛋白质和蛋白质表达的超诱导作用、以及诱导凋亡或促进通过死亡受体诱导的凋亡。他已被证明可以选择性清除动脉粥样硬化斑块中的巨噬细胞、 激活无积垢的马卵母细胞、 并具有神经保护特性。

生化/生理作用

环己酰亚胺(CHX)是由链霉菌属产生的一种抗生素。它的主要生物学活性是真核生物中的翻译抑制,从而导致细胞生长停滞和细胞死亡。CHX被广泛用于抗CHX的酵母和真菌菌株的选择、蛋白质合成的受控抑制以检测短寿命蛋白质和蛋白质表达的超诱导作用、以及诱导凋亡或促进通过死亡受体诱导的凋亡。
环己酰胺可通过与80S核糖体结合而抑制真核细胞中的蛋白质生物合成。

包装

1G,5G

其他说明

安全存放的条件,包括任何不兼容性。保存于密闭容器内,置于干燥通风处。置于干燥处保存。

警示用语:

Danger

危险分类

Acute Tox. 2 Oral - Aquatic Chronic 2 - Muta. 2 - Repr. 1B

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. I know that cycloheximide is effective against molds and yeast. Can Product 01810 also be used as an antibacterial?

    No; cycloheximide is not effective against bacteria.

  4. How do you recommend that solutions of Product 01810, Cycloheximide be stored?

    Solutions of cycloheximide can be stored at room temperature for a few days, but should be kept refrigerated for long-term storage (up to a year). Aqueous solutions at pH 3-5 can be kept refrigerated for several months.

  5. What would you suggest as a working concentration for Product 01810, Cycloheximide?

    The typical working concentration for cycloheximide is 10-100 μg/mL.

  6. What are the differences among Cycloheximide Product Nos. 01810, 01811 and C7698?

    These three cycloheximide products have different purity specifications. Product No. 01810 has a purity specification of at least 93% as determined by HPLC. Product No. 01811 has a purity specification of at least 85% as determined by HPLC. Product No. C7698 has a purity specification of at least 93% as determined by TLC (thin layer chromatography).

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. What is Product 01810, Cycloheximide, soluble in?

    Cyclohexamide is soluble in water up to 20 mg/mL.  Cyclohexamide is also soluble in most organic solvents, including ethanol, with the exception of saturated hydrocarbons.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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The Journal of cell biology, 136(5), 1137-1149 (1997-03-10)
The ability of the protein synthesis inhibitor cycloheximide (CHX) to prevent neuronal death in different paradigms has been interpreted to indicate that the cell death process requires synthesis of "killer" proteins. On the other hand, data indicate that neurotrophic factors
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Ye X, et al.
IUBMB Life, 68(4), 320-327 (2016)
Y H Choi et al.
Reproduction (Cambridge, England), 122(1), 177-183 (2001-06-27)
Two different culture media (TCM-199 and follicular fluid), two activation treatments (10 and 50 micromol calcium ionophore l(-1)) and three culture periods with cycloheximide were evaluated to find effective culture conditions for activation of cumulus-free equine oocytes. Oocytes were collected
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Macrophages are an essential component of unstable atherosclerotic plaques and play a pivotal role in the destabilization process. We have demonstrated previously that local delivery of the mammalian target of rapamycin (mTOR) inhibitor everolimus selectively clears macrophages in rabbit plaques.

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