01673
吖啶黄
BioReagent, suitable for fluorescence, ≥90% (AT)
别名:
中性吖啶黄素, 优黄素, 氯代3,6-二氨基-10-甲基吖啶和3,6-二氨基吖啶（原黄素）混合物
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关于此项目
经验公式（希尔记法）:
C14H14ClN3
化学文摘社编号:
分子量:
259.73
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
MDL number:
InChI
1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H
InChI key
PEJLNXHANOHNSU-UHFFFAOYSA-N
product line
BioReagent
assay
≥90% (AT)
form
powder
composition
Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC
solubility
H2O: 0.33 g/mL (lit.)(lit.)
fluorescence
λex 463 nm; λem 490 nm in ethanol
suitability
suitable for fluorescence
Quality Level
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Application
吖啶黄用于荧光标记高分子量 RNA 分子 。
各种溶剂中的激发和发射波长 ：
不溶于乙醚、氯仿和固定油。在荧光稳态测量中用作供体分子（当与罗丹明 6G 作为受体配对时），起到 pH 传感器的作用 。
各种溶剂中的激发和发射波长 ：
- 甲醇：λex = 424 nm；λem = 518 nm
- 乙醇：λex = 426 nm；λem = 524 nm
- 丙醇：λex = 430 nm；λem = 512 nm
- 丁醇：λex = 430 nm；λem = 526 nm
- 甲酰胺：λex = 434 nm；λem = 524 nm
- 甘油：λex = 432 nm；λem = 540 nm
- 水：λex = 416 nm；λem = 514 nm
不溶于乙醚、氯仿和固定油。在荧光稳态测量中用作供体分子（当与罗丹明 6G 作为受体配对时），起到 pH 传感器的作用 。
Other Notes
DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
M Stöhr et al.
Stain technology, 53(4), 205-215 (1978-07-01)
Eight fluorescent dye combinations for simultaneous DNA-protein staining have been evaluated spectroscopically and flow microfluorometrically: propidium iodide (PI) with fluoresceinisothiocyanate (FITC), fluorescamine (FC), and dansylchloride (DANS); diamidinophenylindole (DAPII) with sulphorhodamin (SR101), tetramethylrhodamin isothiocyanate (TRITC), and nitrobenzodiazole (NBD); acriflavine (AF) with
Zheqing Cai et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(43), 17462-17467 (2013-10-09)
Both preclinical and clinical studies suggest that brief cycles of ischemia and reperfusion in the arm or leg may protect the heart against injury following prolonged coronary artery occlusion and reperfusion, a phenomenon known as remote ischemic preconditioning. Recent studies
Jessica E S Shay et al.
Carcinogenesis, 35(5), 1067-1077 (2014-01-11)
Hypoxia-inducible factors (HIFs) accumulate in both neoplastic and inflammatory cells within the tumor microenvironment and impact the progression of a variety of diseases, including colorectal cancer. Pharmacological HIF inhibition represents a novel therapeutic strategy for cancer treatment. We show here
Nadine Fischer et al.
Biochimica et biophysica acta, 1828(2), 632-641 (2012-10-24)
Acriflavine resistance protein B acts as the active transporter in the multi-drug efflux pump Acriflavine resistance proteins A / B - Tolerance to colicins protein in Escherichia coli. Within the same reaction cycle intermediate all Acriflavine resistance protein B X-ray
Ivan Gusarov et al.
Science (New York, N.Y.), 325(5946), 1380-1384 (2009-09-12)
Bacterial nitric oxide synthases (bNOS) are present in many Gram-positive species and have been demonstrated to synthesize NO from arginine in vitro and in vivo. However, the physiological role of bNOS remains largely unknown. We show that NO generated by
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