产品线
BioReagent
质量水平
检测方案
≥90% (AT)
形式
powder
组成
Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC
溶解性
H2O: 0.33 g/mL (lit.)(lit.)
荧光
λex 463 nm; λem 490 nm in ethanol
适用性
suitable for fluorescence
SMILES字符串
[Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46
InChI
1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H
InChI key
PEJLNXHANOHNSU-UHFFFAOYSA-N
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应用
吖啶黄用于荧光标记高分子量 RNA 分子 。
各种溶剂中的激发和发射波长 ：
不溶于乙醚、氯仿和固定油。在荧光稳态测量中用作供体分子（当与罗丹明 6G 作为受体配对时），起到 pH 传感器的作用 。
各种溶剂中的激发和发射波长 ：
- 甲醇：λex = 424 nm；λem = 518 nm
- 乙醇：λex = 426 nm；λem = 524 nm
- 丙醇：λex = 430 nm；λem = 512 nm
- 丁醇：λex = 430 nm；λem = 526 nm
- 甲酰胺：λex = 434 nm；λem = 524 nm
- 甘油：λex = 432 nm；λem = 540 nm
- 水：λex = 416 nm；λem = 514 nm
不溶于乙醚、氯仿和固定油。在荧光稳态测量中用作供体分子（当与罗丹明 6G 作为受体配对时），起到 pH 传感器的作用 。
其他说明
DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously
警示用语：
Warning
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
M Stöhr et al.
Stain technology, 53(4), 205-215 (1978-07-01)
Eight fluorescent dye combinations for simultaneous DNA-protein staining have been evaluated spectroscopically and flow microfluorometrically: propidium iodide (PI) with fluoresceinisothiocyanate (FITC), fluorescamine (FC), and dansylchloride (DANS); diamidinophenylindole (DAPII) with sulphorhodamin (SR101), tetramethylrhodamin isothiocyanate (TRITC), and nitrobenzodiazole (NBD); acriflavine (AF) with
Zheqing Cai et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(43), 17462-17467 (2013-10-09)
Both preclinical and clinical studies suggest that brief cycles of ischemia and reperfusion in the arm or leg may protect the heart against injury following prolonged coronary artery occlusion and reperfusion, a phenomenon known as remote ischemic preconditioning. Recent studies
Jessica E S Shay et al.
Carcinogenesis, 35(5), 1067-1077 (2014-01-11)
Hypoxia-inducible factors (HIFs) accumulate in both neoplastic and inflammatory cells within the tumor microenvironment and impact the progression of a variety of diseases, including colorectal cancer. Pharmacological HIF inhibition represents a novel therapeutic strategy for cancer treatment. We show here
Ivan Gusarov et al.
Science (New York, N.Y.), 325(5946), 1380-1384 (2009-09-12)
Bacterial nitric oxide synthases (bNOS) are present in many Gram-positive species and have been demonstrated to synthesize NO from arginine in vitro and in vivo. However, the physiological role of bNOS remains largely unknown. We show that NO generated by
Shizuka Nakayama et al.
Chemical communications (Cambridge, England), 47(16), 4766-4768 (2011-03-15)
The ubiquitous bacterial biofilm regulator, c-di-GMP can form G-quadruplexes at physiological conditions in the presence of some aromatic compounds, such as acriflavine and proflavine. The fluorescence of these compounds is quenched upon c-di-GMP binding and some of the formed c-di-GMP
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