T17917
三(羟甲基)甲基甘氨酸
≥99% (titration), reagent grade, crystalline
别名:
N-三(羟甲基)甲基甘氨酸
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线性分子式:
(HOCH2)3CNHCH2CO2H
化学文摘社编号:
分子量:
179.17
NACRES:
NA.25
UNSPSC Code:
12161700
Beilstein/REAXYS Number:
1937804
MDL number:
产品名称
三(羟甲基)甲基甘氨酸, ≥99% (titration), reagent grade, crystalline
SMILES string
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
biological source
synthetic
grade
reagent grade
assay
≥99% (titration)
form
crystalline
storage condition
(Tightly closed. Dry)
impurities
<0.5% water
color
white
useful pH range
7.4-8.8
pKa (25 °C)
8.1
mp
187 °C
solubility
water: 0.1 M, clear, colorless
cation traces
Pb: ≤5 ppm
storage temp.
room temp
Quality Level
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Application
用于分离低分子量的肽的缓冲剂组分。
Tricine is intended to use for:
- Electrophoresis To separate low-weight peptides and proteins
- Crystallization of proteins of varying pH
- Resuspension of cell pellets
- Enzyme assays
- Tissue culture
Features and Benefits
- Ideal as a Biological buffer for Cell Biology and Biochemical research
- Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)
General description
Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.
Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.
Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.
Other Notes
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in
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