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About This Item
线性分子式:
NH2C(=NH)NH2 · HSCN
CAS号:
分子量:
118.16
Beilstein:
3563461
MDL编号:
UNSPSC代码:
12352107
NACRES:
NA.28
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一般描述
Guanadine thiocyanate (GTC) is a chaotropic agent and strong denaturant that is used in research for protein degredation in vitro. This is useful for DNA or RNA isolation and purification. GTC is a key reagent for inactivation of endogenous DNases and RNase for molecular biology techniques used to isolate nucliec acids from tissues, cells, or viral genomes.
应用
离液剂和强效变性剂;溶解细胞。
Guanidine thiocyanate is commonly used for research:
- to denature proteins in cell lysis buffer
- to isolate and purify RNA from cells and tissues
- to extract DNA from cellular and viral genomes
生化/生理作用
Guanadine thiocyanate is strong protein denaturant commonly combinded with phenol to lyse tissues, cells, and proteins during RNA isolation assay.
特点和优势
- Redi-Dri™ makes working with hygroscopic chemicals easier
- Controls moisture and powder remains free-flowing
- Safer to handle and weigh out for routine use in lab
- Eliminates clumps and maintains product moisture and quality attributes
其他说明
For additional information on our range of Biochemicals, please complete this form.
法律信息
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
补充剂危害
储存分类代码
8B - Non-combustible corrosive hazardous materials
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Martin Roszkowski et al.
Frontiers in cell and developmental biology, 9, 648274-648274 (2021-05-11)
The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells. However, it
Peter L Roberts et al.
Biologicals : journal of the International Association of Biological Standardization, 35(4), 343-347 (2007-05-23)
Virus inactivation by a number of protein denaturants commonly used in gel affinity chromatography for protein elution and gel recycling has been investigated. The enveloped viruses Sindbis, herpes simplex-1 and vaccinia, and the non-enveloped virus polio-1 were effectively inactivated by
Serazhutdin A Abdullaev et al.
Radiation and environmental biophysics, 54(3), 297-304 (2015-05-04)
Investigation of cell-free DNA (cf-DNA) in body fluids, as a potential biomarker for assessing the effect of ionizing radiation on the organism, is of considerable interest. We investigated changes in the contents of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear
商品
Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.
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