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一般描述
Tris buffer, pH 7.0 is a general buffer that is isotonic and non-toxic in nature due to the presence of tris base. It is suitable for most biochemical and molecular biology applications. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.
应用
Tris buffer is used in:
- Western blot
- Protein gel electrophoresis
- cell culture (sterile filter buffer)
- As a buffer component
特点和优势
- Fast, ready-to-use buffer
- No need to measure and adjust the pH
- Saves time and minimizes effort in the lab
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
包装
Foil pouches
重悬
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.
储存及稳定性
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
其他说明
For additional information on our range of Biochemicals, please complete this form.
法律信息
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 1
法规信息
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