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产品线
pHast Pack™
质量水平
表单
powder
储存条件
(Tightly closed. Dry)
杂质
≤5 ppm Heavy Metals (lead)
颜色
white to off-white
pH值（酸碱度）
8.3
溶解性
soluble, clear, colorless to faintly yellow
痕量阳离子
Fe: <10 ppm
异质活性
DNase, none detected
Endonuclease
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected
储存温度
room temp
一般描述
Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.
应用
Suitable for use as:
- Transfer buffer component in western blot
- Running buffer in native gel electrophoresis for protein separation
- Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)
特点和优势
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Saves time and minimizes the effort
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
包装
Foil pouches
重悬
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
储存及稳定性
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
其他说明
For additional information on our range of Biochemicals, please complete this form.
法律信息
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
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