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PPB015

Sigma-Aldrich

Tris-Glycine Buffer

pH 8.3, pHast Pack™, powder

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别名:
1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
UNSPSC代码:
41105319
NACRES:
NA.25

产品线

pHast Pack™

质量水平

100

形式

powder

pH值（酸碱度）

8.3

相关类别

一般描述

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

应用

Suitable for use as:
  • Transfer buffer component in western blot
  • Running buffer in native gel electrophoresis for protein separation
  • Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

特点和优势

  • Ready-to-use buffer
  • No measuring and pH adjusting needed
  • Saves time and minimizes the effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包装

Foil pouches

重悬

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

储存及稳定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他说明

For additional information on our range of Biochemicals, please complete this form.

法律信息

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 1

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