跳转至内容
Merck
CN
所有图片(4)

文件

安全信息

PPB008

Sigma-Aldrich

TRIS醋酸盐-EDTA缓冲液

pH 8.3, pHast Pack™, powder

登录查看公司和协议定价
所有图片(4)
别名:
TAE缓冲液
MDL编号:
MFCD00236357
UNSPSC代码:
12352200
NACRES:
NA.21

产品线

pHast Pack™

形式

powder

pH值（酸碱度）

8.3

适用性

suitable for gel electrophoresis (after dilution to working concentration)
suitable for gel electrophoresis

异质活性

DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected

SMILES字符串

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

Tris Acetate-EDTA (TAE) buffer, with a pH range of 8.1 – 8.4, is typically used for agarose gel electrophoresis, especially for the recovery of DNA. For larger double-stranded DNA fragments (>12kB) TAE buffer is preferred. However, the buffering capacity can become exhausted if electrophoresis time is extended. Buffer circulation or replacement during electrophoresis can remedy the lower buffering capacity.

应用

Suitable as a running and gel preparation buffer in:
  • DNA agarose gel electrophoresis
  • Non-denaturing RNA agarose gel electrophoresis for RNA >1500 bases
  • Denaturing gradient gel electrophoresis
  • Northern and Southern blotting
TAE流动缓冲液是DNA琼脂糖凝胶电泳最常用的缓冲液，也可用于非变性RNA琼脂糖凝胶电泳。 双链DNA在TAE中比在其他缓冲液中运行更快，但也可能在延长的电泳过程中耗尽。 在延长的电泳过程中，缓冲液循环或缓冲液更换可以弥补较低的缓冲容量。 浓缩TAE缓冲液稀释后得到1× TAE缓冲液，加入40 mM醋酸三酯和1 mM EDTA，pH 8.3。 1× TAE缓冲液用于琼脂糖凝胶和作为流动缓冲液。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。

特点和优势

  • Ready for use – saves time and effort
  • No measuring and pH adjusting needed
  • Eliminate exposure to toxic chemicals to prepare buffers
  • Biological tests: free of DNase, RNase, Protease, and Nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包装

Foil pouches

制备说明

由经过生物技术性能认证的Trizma碱(T6066)和分子生物学试剂EDTA二钠盐(E5134)制备而成。溶液也含有醋酸(A6283)；粉状混合物含Trizma醋酸(T1258)。

重悬

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 40mM Tris-Acetate, 1mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis.

储存及稳定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他说明

For additional information on our range of Biochemicals, please complete this form.

法律信息

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门