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产品名称
Tris 缓冲盐水，含Tween ™ 20，pH 8.0, powder, pHast Pack™
产品线
pHast Pack™
表单
powder
储存条件
(Tightly closed. Dry.)
杂质
≤5 ppm Heavy Metals (lead)
颜色
white
pH值（酸碱度）
7.8—8.2
溶解性
soluble, clear to hazy, colorless
痕量阳离子
Fe: ≤10 ppm
异质活性
DNase, none detected
Endonuclease
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNase, none detected
储存温度
room temp
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一般描述
Tris Buffered Saline with 0.05% Tween® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.
应用
Suitable for use as a wash buffer in:
- Western blot
- ELISA
- Immunohistochemistry
特点和优势
- Ready-to-use buffer requires no measurments or pH adjustments
- Saves time, money and effort to get your assays started fast
- Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
- Qualified for use in gel electrophoreis and other molecular biology assays
包装
Foil pouches
重悬
Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.
储存及稳定性
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
其他说明
For additional information on our range of Biochemicals, please complete this form.
法律信息
TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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分析证书(COA)
Lot/Batch Number
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