MDCKII canine MDR1 KO, human MDR1 Expressing Cells

MDCKII - Madin-Darby canine kidney - is a subclone derived from the heterogenous parent line MDCK (ECACC Catalog No. 85011435). MDCKII cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm². This cell line is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.

ATP-binding cassette (ABC) transporters are a family of transmembrane proteins that utilize ATP hydrolysis for translocation of substrates across membranes. ABC transporters are known to play a critical role in the development of multidrug resistance. Evaluation of membrane transporter pharmacology in drug disposition and drug-drug interactios (DDI) is critical to the pharmaceutical safety evaluations of new drug entities. Selection of the targeted gene(s) was based on the considerable body of evidence supporting its crucial role in the development of multidrug resistance.

These cells can be used in transwell permeability assays to measure the movement of substrates through the monolayer. Efflux ratios for test compounds can be determined using standard compounds as controls.

CompoZr^® zinc finger nuclease (ZFN) technology is a fast and reliable way to manipulate the genome in a targeted fashion. ZFNs are naturally occurring proteins that can be engineered to bind DNA at a sequence-specific location and create a double strand break. The cell′s natural machinery repairs the break in one of two ways: non-homologous end joining or homologous recombination. The non-homologous end joining pathway typically produces small modifications (indels) at the targeted locus that may result in a functional knockout. Single cell clones are then isolated, tested for the desired modification and expanded to establish a stable cell line.

The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Validation studies have shown no efflux of standard cP-gp substrates.

The human MDR1 efflux transporter has been expressed in these cells via viral transduction. Validation studies have shown efflux ratios ≥2 for common MDR1 substrates digoxin, erythromycin, and quinidine.

Tested for Mycoplasma, sterility, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification

These products are covered by the ADME/Tox Cell Lines License as described in Exhibit 1, in the technical bulletin.

MDCKII sublone was originally isolated by Daniel Louvard, Institut Curie, Paris France.
References: Hansson, G.G., Simons, K and Van Meer G (1986) EMBO 5: 483-489, Louvard D (1980) PNAS 77; 4132-4136

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