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Merck
CN

G3632

庆大霉素 硫酸盐

potency: ≥590 I.U. Gentamicin base per mg

别名:

GM 硫酸盐, 庆大霉素 C

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化学文摘社编号:
MDL number:
UNSPSC Code:
51281651
eCl@ss:
42020823
NACRES:
NA.76
EC Number:
215-778-9
PubChem Substance ID:
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产品名称

庆大霉素 硫酸盐, potency: ≥590 I.U. Gentamicin base per mg

InChI

1S/C21H43N5O7.C20H41N5O7.C19H39N5O7.H2O4S/c1-9(25-3)13-6-5-10(22)19(31-13)32-16-11(23)7-12(24)17(14(16)27)33-20-15(28)18(26-4)21(2,29)8-30-20;1-8(21)12-5-4-9(22)18(30-12)31-15-10(23)6-11(24)16(13(15)26)32-19-14(27)17(25-3)20(2,28)7-29-19;1-19(27)7-28-18(13(26)16(19)24-2)31-15-11(23)5-10(22)14(12(15)25)30-17-9(21)4-3-8(6-20)29-17;1-5(2,3)4/h9-20,25-29H,5-8,22-24H2,1-4H3;8-19,25-28H,4-7,21-24H2,1-3H3;8-18,24-27H,3-7,20-23H2,1-2H3;(H2,1,2,3,4)/t9-,10-,11+,12-,13+,14+,15-,16-,17+,18-,19-,20-,21+;8-,9-,10+,11-,12+,13+,14-,15-,16+,17-,18-,19-,20+;8-,9+,10-,11+,12-,13+,14+,15-,16+,17+,18+,19-;/m110./s1

InChI key

RDEIXVOBVLKYNT-HDZPSJEVSA-N

SMILES string

O=S(O)(O)=O.O[C@]1(C)[C@@H]([C@@H](O)[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@H]3O[C@@](CC[C@H]3N)([C@@H](C)NC)[H])[C@@H](N)C[C@H]2N)OC1)NC.O[C@]4(C)[C@@H]([C@@H](O)[C@@H](O[C@@H]5[C@@H](O)[C@H](O[C@H]6O[C@@](CC[C@H]6N)([C@@H](C)N)[H])[C@@H](N)C[C@H]5N)OC4)NC.O[

biological source

Micromonospora purpurea

form

powder

potency

≥590 I.U. Gentamicin base per mg
≥590 units/mg Gentamicin

storage condition

(Tightly closed. Dry. )

concentration

50 μg/mL in H2O (is the recommended working concentration for eukaryotic cell culture)

color

white to off-white

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycoplasma

mode of action

protein synthesis | interferes

storage temp.

2-8°C

Quality Level

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Preparation Note

无菌溶液应储存在2-8°C条件下。溶液在室温及沸腾的pH 2-14的水性缓冲液中稳定。[2]

General description

硫酸庆大霉素盐是一种广谱氨基糖苷类抗生素,衍生自紫癜性小单孢子菌。

Other Notes

如需了解生化试剂系列的更多信息,请填写此表
庆大霉素是Micromonospora purpurea M. echinospora发酵后产生的氨基糖苷复合物。 它可以用来作为硫酸盐。 每摩尔庆大霉素基础有三个成分,每一个成分包含五份碱性氮,并且需要5份硫酸。
易溶于水,几乎不溶于乙醇

Application

硫酸庆大霉素在细胞培养和分子生物实验中作为选择剂(抗庆大霉素基因)。 产品推荐使用浓度是50mg/mL。 硫酸庆大霉素可用于:
  • 抗生素对不同细菌培养物的有效性研究中
  • 研究神经元信号处理

Biochem/physiol Actions

作用机制:庆大大霉素通过和30S核糖亚基结合,引起密码子误读,阻碍肽酰转移酶从供体位点转移到受体位点。庆大霉素通过它与外膜的结合来发挥它对铜绿假单胞菌的杀菌作用,它在外膜上置换天然阳离子,使膜不稳定,并在细胞表面形成孔。

抗菌谱:包括革兰氏阴性和革兰氏阳性细菌,包括对于四环素、氯霉素、卡那霉素和粘菌素,某些特定假单胞菌菌株,变形杆菌属,StaphylococcusStreptococcus 的抗性。

Features and Benefits

适合多种研究应用的高质量抗生素

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

涉药品监管产品
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分析证书(COA)

Lot/Batch Number

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The effect of ultrasonic frequency upon enhanced killing of P. aeruginosa biofilms
Qian et al.
Annals of Biomedical Engineering, 25(1):69-25(1):76 (1997)
J J Whyte et al.
Transgenic research, 20(5), 989-1001 (2010-12-21)
The objective of this study was to develop transgenic Yucatan minipigs that overexpress human catalase (hCat) in an endothelial-specific manner. Catalase metabolizes hydrogen peroxide (H(2)O(2)), an important regulator of vascular tone that contributes to diseases such as atherosclerosis and preeclampsia.
Louise Menendez et al.
eLife, 9 (2020-07-01)
The mechanoreceptive sensory hair cells in the inner ear are selectively vulnerable to numerous genetic and environmental insults. In mammals, hair cells lack regenerative capacity, and their death leads to permanent hearing loss and vestibular dysfunction. Their paucity and inaccessibility
W G Pitt et al.
Antimicrobial agents and chemotherapy, 38(11), 2577-2582 (1994-11-01)
The effect of gentamicin upon planktonic cultures of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, and Staphylococcus aureus was measured with and without application of 67-kHz ultrasonic stimulation. The ultrasound was applied at levels that had no inhibitory or bactericidal activity
Mimmi Werner et al.
Anatomical record (Hoboken, N.J. : 2007), 303(3), 506-515 (2019-05-16)
The auditory apparatus of the inner ear does not show turnover of sensory hair cells (HCs) in adult mammals; in contrast, there are many observations supporting low-level turnover of vestibular HCs within the balance organs of mammalian inner ears. This

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